Development of a microsphere-based fluorescence immunochromatographic assay for monitoring lincomycin in milk, honey, beef, and swine urine

Abstract

The residue of lincomycin (LIN) in edible animal foodstuffs caused by the widespread use of veterinary drugs is in need of rapid, simple, and sensitive detection methods. The present work introduces a fluorescent microsphere immunoassay (FMIA) for detecting LIN in different samples based on the competitive immunoreaction on the chromatography test strip. The residues of LIN in different samples compete with bovine serum albumin (BSA) labeled LIN conjugates on the T-line to bind to the anti-LIN monoclonal antibody labeled fluorescent microspheres (FM-mAbs). Captured FM-mAbs on the T-line represent the fluorescent intensity, which is detected under UV light and quantified by a fluorescent reader. Under optimized conditions, the dynamic range is from 1.35 to 3.57 ng/mL, and the 50% inhibition concentration (IC50) is 2.20 ng/mL. This method has 4.4% cross-reactivity with clindamycin and negligible cross-reactivity (<0.1%) with other analogues. To reduce the matrix effects, a dilution method is used to pretreat the samples, and the recoveries range from 73.92 to 120.50% with coefficient of variations <21.76%. In comparison with the results of ELISA and colloidal gold immunoassay, FMIA has obvious advantages such as easy operation, time savings, high sensitivity and specificity, and broader prospect.

Keywords: fluorescent microspheres; immunoassay; lincomycin; residue.