Is there a single differentiation pathway for all antibody-forming cells in the spleen?

Abstract

By incubating tissue sections with antigen (or hapten)-enzyme conjugates, followed by enzyme cytochemistry(1-4), N. van Rooijen and his colleagues have been able to study the development and localization of specific antibody-producing cells in the spleen of animals after a single intravenous antigen injection. Variations in this method(5-11) allowed them to compare the localization patterns of cells producing different antibodies and to determine simultaneously their Ig isotype. Studies on primary and secondary immune responses against thymus-dependent and thymus-independent antigens lead them to postulate that there is one 'differentiation pathway' for antibody-forming cells in the spleen, independent of the antigen and the type of immune response.