Immunoassay of beta-hexosaminidase isoenzymes in serum in patients with raised total activities

Abstract

Enzyme immunoassay (EIA) methods with monoclonal antibodies specific for beta-hexosaminidase (Hex) isoenzymes A and B in human serum are presented. The proportion of Hex A obtained with the EIA-methods was similar to that found with ion-exchange chromatography and isoelectric focusing (about 60% of total Hex activity). The EIA-method for Hex B reacted to a similar extent with Hex B, Hex P and intermediate forms. A highly significant correlation was obtained between total Hex activity in human sera assayed with a conventional enzyme substrate method and the total Hex activity obtained as the sum of Hex A and Hex B analyzed with the EIA-methods. In sera from individuals with increased total activity of Hex there was a positive correlation between total activity of Hex, and percent of Hex B found with the EIA-methods. Investigation of sera from pregnant women and sera from patients with liver cirrhosis and cholestasis showed that the increase in total activity in these patient groups was mainly due to an increase of the isoenzyme forms reacting with the EIA-method for Hex B.