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. 2014 Aug;10(Suppl 3):S456-62.
doi: 10.4103/0973-1296.139773.

Chemical constituents and toxicological studies of leaves from Mimosa caesalpiniifolia Benth., a Brazilian honey plant

Nayana Bruna Nery Monção  1 , Luciana Muratori Costa  1 , Daniel Dias Rufino Arcanjo  2 , Bruno Quirino Araújo  1 , Maria do Carmo Gomes Lustosa  1 , Klinger Antônio da França Rodrigues  2 , Fernando Aécio de Amorim Carvalho  2 , Amilton Paulo Raposo Costa  3 , Antônia Maria das Graças Lopes Citó  1
Affiliations

Chemical constituents and toxicological studies of leaves from Mimosa caesalpiniifolia Benth., a Brazilian honey plant

Nayana Bruna Nery Monção et al. Pharmacogn Mag. 2014 Aug.

Abstract

Background: Mimosa caesalpiniifolia Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region.

Objective: To investigate the chemical composition of the ethanol extract of leaves from M. caesalpiniifolia by GC-MS after derivatization (silylation), as well as to evaluate the in vitro and in vivo toxicological effects and androgenic activity in rats.

Materials and methods: The ethanol extract of leaves from Mimosa caesalpiniifolia was submitted to derivatization by silylation and analyzed by gas chromatography-mass spectrometry (GC-MS) to identification of chemical constituents. In vitro toxicological evaluation was performed by MTT assay in murine macrophages and by Artemia salina lethality assay, and the in vivo acute oral toxicity and androgenic evaluation in rats.

Results: Totally, 32 components were detected: Phytol-TMS (11.66%), lactic acid-2TMS (9.16%), α-tocopherol-TMS (7.34%) and β-sitosterol-TMS (6.80%) were the major constituents. At the concentrations analyzed, the ethanol extract showed low cytotoxicity against brine shrimp (Artemia salina) and murine macrophages. In addition, the extract did not exhibit any toxicological effect or androgenic activity in rats.

Conclusions: The derivatization by silylation allowed a rapid identification of chemical compounds from the M. caesalpiniifolia leaves extract. Besides, this species presents a good safety profile as observed in toxicological studies, and possess a great potential in the production of herbal medicines or as for food consumption.

Keywords: Cytotoxicity; GC-MS; Leguminosae; Mimosa caesalpiniifolia; derivatization; toxicology.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Total ion chromatogram of ethanol extract from leaves of Mimosa caesalpiniifolia (Mc-EtOH) submitted to derivatization (silylation) and analyzed by GC-MS
See this image and copyright information in PMC

References

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