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. 2015 Feb;77(2):221-4.
doi: 10.1292/jvms.14-0379. Epub 2014 Oct 13.

Detection of avian-like rotavirus A VP4 from a calf in Japan

Hiromichi Mitake  1 , Naoto Ito, Kota Okadera, Kazuma Okada, Kento Nakagawa, Tomomi Tanaka, Kiyohito Katsuragi, Kasumi Kasahara, Toshihide Nihongi, Hiroshi Tsunemitsu, Makoto Sugiyama
Affiliations

Detection of avian-like rotavirus A VP4 from a calf in Japan

Hiromichi Mitake et al. J Vet Med Sci. 2015 Feb.

Abstract

A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus.

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Figures

Fig. 1.
Fig. 1.
Phylogenetic tree based on partial VP4 nucleotide sequences (769 bp, corresponding to nucleotides 53-833 of the NCDV VP4 gene) of 37 established reference P genotype strains and N2342 strains. Bootstrap values above 60% are shown at the branch nodes. Reference rotavirus strains and accession numbers for the VP4 genes used to construct the phylogenetic tree are as follows. P[1]: NCDV (AB119636), P[2]: SA11 (M23188), P[3]: RRV (M18736), P[4]: L26 (M58292), P[5]: UK (M22306), P[6]: Gottfried (M33516), P[7]: OSU (X13190), P[8]: Wa (L34161), P[9]: AU-1 (D10970), P[10]: 69M (M60600), P[11]: B223 (D13394), P[12]: H-2 (L04638), P[13]: MDR-13 (L07886), P[14]: Sun9 (AB158430), P[15]: Lp14 (L11599), P[16]: EDIM (AF039219), P[17]: PO-13 (AB009632), P[17]: 993/83 (D16352), P[18]: L338 (D13399), P[19]: Mc345 (D38054), P[20]: EHP (U08424), P[21]: Hg18 (AF237665), P[22]: 160/01 (AF526374), P[23]: 34461-4 (AY768809), P[24]: TUCH (AY596189), P[25]: Dhaka6 (AY773004), P[26]: 134/04-15 (DQ061053), P[27]: CMP034 (DQ534016), P[28]: Ecu534 (EU805773), P[29]: AzuK-1 (AB454420), P[30]: Ch-2G3 (EU486956), P[31]: Ch-661G1 (EU486962), P[32]: 61-07-ire (FJ492835), P[33]: Dai-10 (AB513836), P[34]: FGP51 (AB571047), P[35]: 03V0002E10 (JX204825), P[36]: SG385 (AB823215), P[37]: 10V0112H5 (JX204814). Bo, bovine; Ch, chicken; Eq, equine; Hu, human; La, lapine; Mu, murine; Ov, ovine; Ph, pheasant; Pi, pigeon; Po, porcine; SG, sugar glider; Si, simian; Tu, turkey; WB, wild boar.
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