Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;18(12):2553-7.
doi: 10.1111/jcmm.12411. Epub 2014 Oct 14.

Decreased expression of KGF/FGF7 and its receptor in pathological hypopigmentation

Valeria Purpura  1 , Flavia Persechino, Francesca Belleudi, Cristina Scrofani, Salvatore Raffa, Severino Persechino, Maria Rosaria Torrisi
Affiliations

Decreased expression of KGF/FGF7 and its receptor in pathological hypopigmentation

Valeria Purpura et al. J Cell Mol Med. 2014 Dec.
No abstract available

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Decreased expression and release of KGF from hypopigmentary lesional fibroblasts leads to reduced melanosome transfer. (A) Cocultures of MST-L melanoma cells and HaCaT keratinocytes were stimulated with KGF or with SNs (undiluted or diluted 1:2 or 1:5) from NHFs or lesional HFs. Immunofluorescence shows a significant decrease of the fluorescent tyrosinase-positive dots, corresponding to transferred melanosomes, in the pancytokeratin-positive keratinocytes upon stimulation with SNs from lesional HFs with respect to the treatment with the SN from NHFs or with KGF. The KGFR inhibitor SU5402 blocks the melanosome uptake. Quantitation of tyrosinase fluorescence intensity and Student's t-test were performed as reported in Data S1; bars: 10 μm. (B) Real-time RT-PCR reveals a decreased KGF mRNA expression in HFs from the lesional samples compared with the control NHFs. (C) Quantitation of the released KGF protein by ELISA test performed on the SNs shows that KGF levels in the SNs from lesional samples are significantly decreased with respect to control fibroblasts. Results represent the mean values ± SD. Mann–Whitney test was performed and significance level has been defined as described in Data S1. (D) MTT test shows that none of the treatments with SNs is cytotoxic for the cells up to 48 hrs. Results represent the mean values ± SD and Student's t-test was performed as reported in Data S1.
Fig. 2
Fig. 2
Decreased melanosome uptake ability and KGFR expression in keratinocytes from ND lesion. (A and B) Cocultures of MST-L melanoma cells with normal human keratinocytes (NHKs) or with keratinocytes derived from the ND lesion (ND HKs) were treated with KGF. Immunofluorescence (A and B) and phase-contrast (B) images show that the tyrosinase-positive dots in ND HKs upon KGF treatment are strongly reduced with respect to those in NHKs (A and B, circles) and that the addition of SU5402 abolishes the KGF effect; bars: 10 μm. (C) Real-time RT-PCR reveals a decreased KGFR mRNA expression in ND HKs compared with NHK control cells. (D) Schematic drawing showing the effects of decreased levels of KGF and KGFR on melanosome transfer in hypopigmented lesions.
See this image and copyright information in PMC

References

    1. Seiberg M, Paine C, Sharlow E, et al. The protease-activated receptor-2 regulates pigmentation via keratinocyte-melanocyte interactions. Exp Cell Res. 2000;254:25–32. - PubMed
    1. Cardinali G, Ceccarelli S, Kovacs D, et al. Keratinocyte growth factor promotes melanosome transfer to keratinocytes. J Invest Dermatol. 2005;125:1190–9. - PubMed
    1. Van Den Bossche K, Naeyaert JM, Lambert J. The quest for the mechanism of melanin transfer. Traffic. 2006;7:1–10. - PubMed
    1. Cardinali G, Bolasco G, Aspite N, et al. Melanosome transfer promoted by keratinocyte growth factor in light and dark skin-derived keratinocytes. J Invest Dermatol. 2008;128:558–67. - PubMed
    1. Belleudi F, Purpura V, Scrofani C, et al. Expression and signaling of the tyrosine kinase FGFR2b/KGFR regulates phagocytosis and melanosome uptake in human keratinocytes. FASEB J. 2011;25:170–81. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources