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. 2014:2014:581890.
doi: 10.1155/2014/581890. Epub 2014 Sep 17.

In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

S Rajeshkumar  1 , C Malarkodi  2
Affiliations

In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

S Rajeshkumar et al. Bioinorg Chem Appl. 2014.

Abstract

Biosynthesis of silver nanoparticles using Planomicrobium sp. and to explore the antibacterial activity against food borne pathogenic bacteria Bacillus subtilis, (3053) Klebsiella planticola (2727) Klebsiella pneumoniae (MAA) Serratia nematodiphila (CAA) and Escherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL of Planomicrobium sp. culture supernatant. The bioreduction of pure AgNO3 was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

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Figures

Figure 1
Figure 1
Biosynthesis of silver nanoparticles (a) and culture supernatant of Planomicrobium sp. (b) after the addition of AgNO3 at 24 hrs incubation.
Figure 2
Figure 2
UV spectra of extracellularly synthesized silver nanoparticles using culture supernatant of Planomicrobium sp. show that the SPR band at 400 nm indicates nanoparticles synthesis.
Figure 3
Figure 3
XRD pattern of extracellular synthesized silver nanoparticles using Planomicrobium sp.
Figure 4
Figure 4
SEM image of silver nanoparticles shows spherical shape with agglomeration at different magnification, (a) 2,000x and (b) 5,000x.
Figure 5
Figure 5
EDX analysis of silver nanoparticles.
Figure 6
Figure 6
TEM image of silver nanoparticles shows spherical shape at 50 nm scale bar; (b) SAED pattern indicates crystalline nature of synthesized silver nanoparticles.
Figure 7
Figure 7
FTIR spectra of silver nanoparticle synthesized by Planomicrobium sp.
Figure 8
Figure 8
Antibacterial activity of silver nanoparticles against pathogens by agar well diffusion method.
Figure 9
Figure 9
Antibacterial activity of silver nanoparticles by broth dilution method: (a) B. subtilis, (b) K. planticola, (c) K. pneumoniae, (d) S. nematodiphila, and (e) E. coli.
Figure 10
Figure 10
Mechanism of antibacterial activity of SNPs against food borne pathogens.
See this image and copyright information in PMC

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