Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Dec 8;246(4935):1275-81.
doi: 10.1126/science.2531466.

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

W D Huse  1 , L Sastry, S A Iverson, A S Kang, M Alting-Mees, D R Burton, S J Benkovic, R A Lerner
Affiliations

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

W D Huse et al. Science. .

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

PubMed Disclaimer

Comment in

Publication types

MeSH terms

Substances

LinkOut - more resources