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. 2015 Feb:178:254-261.
doi: 10.1016/j.biortech.2014.08.111. Epub 2014 Oct 2.

Augmented biogas production from protein-rich substrates and associated metagenomic changes

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Free article

Augmented biogas production from protein-rich substrates and associated metagenomic changes

Etelka Kovács et al. Bioresour Technol. 2015 Feb.
Free article

Abstract

This study demonstrates that appropriate adaptation of the microbial community to protein-rich biomass can lead to sustainable biogas production. The process of acclimation to these unusual mono-substrates was controlled by the protease activity of the microbial community. Meat extract (C/N=3.32) and kitchen waste (C/N=12.43) were used as biogas substrates. Metagenome analysis highlighted several mesophilic strains that displayed a preference for protein degradation. Bacillus coagulans, Bacillus subtilis and Pseudomonas fluorescens were chosen for detailed investigation. Pure cultures were added to biogas reactors fed solely with protein-rich substrates. The bioaugmentation resulted in a 50% increase in CH4 production even without any acclimation. The survival and biological activity of the added bacteria were followed in fed-batch fermenters by qPCR. Stable biogas production was observed for an extended period of time in laboratory CSTR reactors fed with biomass of low C/N.

Keywords: Anaerobic degradation; Bioaugmentation; Biogas; Metagenomics; Protein-rich waste.

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