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Review
. 2015 Mar;43(3):681-96.
doi: 10.1007/s10439-014-1151-0. Epub 2014 Oct 16.

Pre-clinical characterization of tissue engineering constructs for bone and cartilage regeneration

Jordan E Trachtenberg  1 , Tiffany N Vo, Antonios G Mikos
Affiliations
Review

Pre-clinical characterization of tissue engineering constructs for bone and cartilage regeneration

Jordan E Trachtenberg et al. Ann Biomed Eng. 2015 Mar.

Abstract

Pre-clinical animal models play a crucial role in the translation of biomedical technologies from the bench top to the bedside. However, there is a need for improved techniques to evaluate implanted biomaterials within the host, including consideration of the care and ethics associated with animal studies, as well as the evaluation of host tissue repair in a clinically relevant manner. This review discusses non-invasive, quantitative, and real-time techniques for evaluating host-materials interactions, quality and rate of neotissue formation, and functional outcomes of implanted biomaterials for bone and cartilage tissue engineering. Specifically, a comparison will be presented for pre-clinical animal models, histological scoring systems, and non-invasive imaging modalities. Additionally, novel technologies to track delivered cells and growth factors will be discussed, including methods to directly correlate their release with tissue growth.

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Conflict of interest statement

Conflict of interest: No benefits in any form have been or will be received from a commercial party related directly or indirectly to the subject of this manuscript.

References

    1. Abdulreda MH, Faleo G, Molano RD, Lopez-Cabezas M, Molina J, Tan Y, Echeverria OA, Zahr-Akrawi E, Rodriguez-Diaz R, Edlund PK, Leibiger I, Bayer AL, Perez V, Ricordi C, Caicedo A, Pileggi A, Berggren PO. High-resolution, noninvasive longitudinal live imaging of immune responses. Proc. Natl. Acad. Sci. U. S. A. 2011;108:12863–12868. - PMC - PubMed
    1. Allen A, Gazit Z, Su S, Stevens H, Guldberg RE. In vivo bioluminescent tracker of mesechymal stem cells within large hydrogel constructs. Tissue Eng. Part C. 2014;20:1–11. - PMC - PubMed
    1. An YH, Draughn RA. Mechanical testing of bone and the bone-implant interface. 1 ed. Boca Raton, FL: CRC Press; 1999. p. 624.
    1. Appel AA, Anastasio MA, Larson JC, Brey EM. Imaging challenges in biomaterials and tissue engineering. Biomaterials. 2013;34:6615–6630. - PMC - PubMed
    1. Artzi N, Oliva N, Puron C, Shitreet S, Artzi S, bon Ramos A, Groothuis A, Sahagian G, Edelman ER. In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging. Nat Mater. 2011;10:704–709. - PMC - PubMed

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