Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Oct;20(4):411-23.
doi: 10.1007/s12298-014-0250-6. Epub 2014 Jul 5.

Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers

Md Nasim Ali  1 , Lucina Yeasmin  1 , Saikat Gantait  2 , Rupak Goswami  1 , Somsubhra Chakraborty  1
Affiliations

Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers

Md Nasim Ali et al. Physiol Mol Biol Plants. 2014 Oct.

Abstract

The present investigation was carried out to evaluate 33 rice landrace genotypes for assessment of their salt tolerance at seedling stage. Growth parameters like root length, shoot length and plant biomass were measured after 12 days of exposure to six different levels of saline solution (with electrical conductivity of 4, 6, 8, 10, 12 or 14 dS m (-1)). Genotypes showing significant interaction and differential response towards salinity were assessed at molecular level using 11 simple sequence repeats (SSR) markers, linked with salt tolerance quantitative trait loci. Shoot length, root length and plant biomass at seedling stage decreased with increasing salinity. However, relative salt tolerance in terms of these three parameters varied among genotypes. Out of the 11 SSR markers RM8094, RM336 and RM8046, the most competent descriptors to screen the salt tolerant genotypes with higher polymorphic information content coupled with higher marker index value, significantly distinguished the salt tolerant genotypes. Combining morphological and molecular assessment, four lanraces viz. Gheus, Ghunsi, Kuthiahara and Sholerpona were considered as true salt tolerant genotypes which may contribute in greater way in the development of salt tolerant genotypes in rice.

Keywords: Morphological descriptor; Oryza sativa L; SSR marker; Salt tolerance.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Effect of different salinity level on a. shoot length, b. root length and c. plant biomass at 12 days after exposure to saline nutrient solution for 33 rice genotypes. Error bars represent standard error of the mean of three replicates
Fig. 2
Fig. 2
Distribution of genotypes under study in 3-dimensitonal space of morphological salinity indices at seedling stage at a. EC-4, b. EC-6, c. EC-8, d. EC-10, e. EC-12 and f. EC-14 salinity levels
Fig. 3
Fig. 3
Clusters of genotypes under study using Euclidian distance matrix at a. EC-4, b. EC-6, c. EC-8, d. EC-10, e. EC-12 and f. EC-14
Fig. 4
Fig. 4
Polyacrylamide gel electrophoresis of SSR fragments (generated by primer RM 8094) of 33 rice genotypes under study where Lane L- 50 bp ladder, 1 = Sholerpona, T = Tolerant check (Talmugur), 3 = Kalobhog, 4 = Sabitapalui, 5 = Kumrogor, 6 = Taksal, 7 = Gheus, 8 = Kuthiahara, 9 = Barsa, 10 = Ansapat, 11 = patnai, Pankaj, 13 = Bhelkibagra, 14 = Sadamota, 16 = Chamarmuni, 17 = Gobindabhog, 18 = Dudheswar, 19 = Langalmura, 20 = Kapur Dhul, 21 = Tulaipanji, 22 = Kanakchur, 23 = Kanakchur, 24 = Thavallakanan, 25 = Shyamali, 26 = Hiramoti, 27 = Annapurna, 28 = Anjali, 29 = Malabati, 30 = Kaminibhog, 31 = Ramshal, 32 = Marichshal and 33 = Kataribhog
Fig. 5
Fig. 5
Dendrogram based on SSR banding profile among 33 genotypes of rice under study
See this image and copyright information in PMC

References

    1. Akbar M, Khush GS, HilleRisLambers D. Genetics of salt tolerance in rice. Proceedings of the Rice Genetics. Manila, Philippines: International Rice Research Institute; 1986. pp. 399–409.
    1. Aliyu R, Adamu AK, Muazu S, Alonge SO, Gregorio GB. Tagging and Validation of SSR markers to Salinity Tolerance QTLs in Rice (Oryza spp) Singapore: 2010 International Conference on Biology, Environment and Chemistry, IPCBEE, IACSIT press; 2011. pp. 328–332.
    1. ANU (2006) Technological Transformation of Productivity, Profitability and Sustainability: Rice. In The First Ten K R Narayanan Orations: Essays by Eminent Persons on the Rapidly Transforming Indian Economy. Australia South Asia Research Centre The Australian National University. http://epress.anu.edu.au/narayanan/mobile_devices/pr01.html
    1. Beyene Y, Botha AM, Myburg AA. A comparative study of molecular and morphological methods of describing genetic relationships in traditional Ethiopian highland maize. Afr J Biotechnol. 2005;4:586–595.
    1. Bhuiyan MAR. Efficiency in evaluating salt tolerance in rice using phenotypic and marker assisted selection. M.Sc. dissertation. Bangladesh: Department of Genetics and Plant Breeding, Bangladesh Agricultural University; 2005. p. 96.

LinkOut - more resources