Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015:1229:549-55.
doi: 10.1007/978-1-4939-1714-3_43.

Heparan sulfate modulates Slit3-induced endothelial cell migration

Hong Qiu  1 , Wenyuan Xiao, Jingwen Yue, Lianchun Wang
Affiliations

Heparan sulfate modulates Slit3-induced endothelial cell migration

Hong Qiu et al. Methods Mol Biol. 2015.

Abstract

Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

PubMed Disclaimer

Publication types

LinkOut - more resources