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Review
. 2015 Feb;37(2):265-79.
doi: 10.1007/s10529-014-1699-7. Epub 2014 Oct 18.

Recent developments in therapeutic protein expression technologies in plants

Shah Fahad  1 , Faheem Ahmed Khan, Nuruliarizki Shinta Pandupuspitasari, Muhammad Mahmood Ahmed, Yu Cai Liao, Muhammad Tahir Waheed, Muhammad Sameeullah, Darkhshan, Saddam Hussain, Shah Saud, Shah Hassan, Amanullah Jan, Mohammad Tariq Jan, Chao Wu, Ma Xiao Chun, Jianliang Huang
Affiliations
Review

Recent developments in therapeutic protein expression technologies in plants

Shah Fahad et al. Biotechnol Lett. 2015 Feb.

Abstract

Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.

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Figures

Fig. 1
Fig. 1
Key events in the development of plant-derived biopharmaceuticals
See this image and copyright information in PMC

References

    1. Azhakanandam K, Weissinger SM, Nicholson JS, Qu R, Weissinger AK. Amplicon-plus targeting technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants. Plant Mol Biol. 2007;63:393–404. - PubMed
    1. Bendich AJ. Why do chloroplasts and mitochondria contain so many copies of their genome? BioEssays. 1987;6:279–282. - PubMed
    1. Boothe J, Nykiforuk C, Shen Y, Zaplachinski S, Szarka S, Kuhlman P, Murray E, Morck D, Moloney MM. Seed-based expression systems for plant molecular farming. Plant Biotechnol J. 2010;8:588–606. - PubMed
    1. Broothaerts W, Mitchell HJ, Weir B, Kaines S, Smith LMA, Yang W, Maye JE, Roa-Rodríguez C, Jefferson RA. Gene transfer to plants by diverse species of bacteria. Nature. 2005;433:629–633. - PubMed
    1. Buchmann RC, Asad S, Wolf JN, Mohannath G, Bisaro DM. Geminivirus AL2 and L2 proteins suppress transcriptional gene silencing and cause genome-wide reductions in cytosine methylation. J Virol. 2009;83:5005–5013. - PMC - PubMed

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