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. 2014 Oct;51(10):2490-8.
doi: 10.1007/s13197-012-0720-1. Epub 2012 Jul 7.

Process optimization for enzyme aided clarification of watermelon juice

Deepali Saxena  1 , Latha Sabikhi  2 , Subir Kumar Chakraborty  1 , Dheer Singh  1
Affiliations

Process optimization for enzyme aided clarification of watermelon juice

Deepali Saxena et al. J Food Sci Technol. 2014 Oct.

Abstract

Watermelon juice was exposed to the enzyme masazyme at varying enzyme concentrations (0.01-0.1 % w/w) and different time (20-120 min) and temperature (30-50 °C) combinations. The effects of the treatments on selected responses (juice recovery, total dissolved solids (TDS), viscosity, turbidity, cloud stability and L value) were determined employing a second order Box Behnken Design in combination with Response Surface Methodology. Enzymatic treatment effectively degraded polysaccharides, resulting in reduced viscosity, turbidity and absorbance value and increased juice recovery, total dissolved solids and lightness. R(2) value for all models for the dependent variables were greater than 90 %. The maximum juice recovery (86.27 %), TDS (8.7°Brix) and L value (17.57) while minimum viscosity (0.0020 Pa.s.), turbidity (39.37 NTU) and cloud stability (0.033 abs) were obtained when enzyme treatment was set up with 0.09 % w/w enzyme concentration at 46.90 °C and 117.45 min.

Keywords: Enzyme; Juice recovery; Optimization; Turbidity; Watermelon.

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Figures

Fig.1
Fig.1
Response surface plots for different parameters as a function of enzyme concentration, incubation time and incubation temperature
Fig. 2
Fig. 2
Superimposed diagram for optimum condition as a function of enzyme concentration and incubation time at 46.90 °C
See this image and copyright information in PMC

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References

    1. Abdullah LAG, Sulaiman NM, Aroua MK, Megat Mohd Noor MJ. Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme. J Food Eng. 2007;81(1):65–71. doi: 10.1016/j.jfoodeng.2006.10.013. - DOI
    1. Ahmad I, Jha YK, Anurag RK. Optimization of enzymic extraction process for higher yield and clarity of guava juice. J Food Sci Technol. 2009;46(4):307–311.
    1. Alvarez S, Alvarez R, Riera FA, Coca J. Influence of depectinization on apple juice ultrafiltration. Colloids Surf A. 1998;138(2–3):377–382. doi: 10.1016/S0927-7757(98)00235-0. - DOI
    1. Chakraborty SK, Kumbhar BK, Chakraborty S, Yadav P. Influence of processing parameters on textural characteristics and overall acceptability of millet enriched biscuits using response surface methodology. J Food Sci Technol. 2011;48(2):167–174. doi: 10.1007/s13197-010-0164-4. - DOI - PMC - PubMed
    1. Chang TS, Siddiq M, Sinha NK, Cash JN. Commercial pectinase and the yield quality of stanley plum juice. J Food Process Preserv. 1995;19(2):89–101. doi: 10.1111/j.1745-4549.1995.tb00280.x. - DOI

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