Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 May 27:Suppl 6:002.
doi: 10.4172/2155-6113.S6-002.

The Use of Ultra-Sensitive Molecular Assays in HIV Cure-Related Research

Affiliations

The Use of Ultra-Sensitive Molecular Assays in HIV Cure-Related Research

Catherine Kibirige. J AIDS Clin Res. .

Abstract

Ultra-sensitive laboratory assays based on the Polymerase Chain Reaction (PCR) are playing an increasingly important role in HIV cure-related research. This article reviews the different assays available and how they have evolved. There is a great need for their standardization and for the establishment of reference reagents and testing algorithms to evaluate potential HIV cure-related treatments.

Keywords: 2-LTR; Eradication; HIV; Nucleic acid tests; PCR; Proviral DNA; Standardization.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Rosenberg ES, Brennan CA, Claessens C, Constantine NT, Murphy G, et al. Criteria for Laboratory Testing and Diagnosis of Human Immunodeficiency Virus Infection; Approved Guideline. Vol. 31. Clinical and Laboratory Standards Institute; Wayne, Pennsylvania: 2011. pp. 1–89.
    1. Lewin SR, Rouzioux C. HIV cure and eradication: how will we get from the laboratory to effective clinical trials? AIDS. 2011;25:885–897. - PubMed
    1. Eisele E, Siliciano RF. Redefining the viral reservoirs that prevent HIV-1 eradication. Immunity. 2012;37:377–388. - PMC - PubMed
    1. Li P, Ruel T, Fujimoto K, Hatano H, Yukl S, et al. Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes. J Virol Methods. 2010;170:115–120. - PMC - PubMed
    1. Casabianca A, Gori C, Orlandi C, Forbici F, Federico Perno C, et al. Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay. Mol Cell Probes. 2007;21:368–378. - PubMed

LinkOut - more resources