The Use of Ultra-Sensitive Molecular Assays in HIV Cure-Related Research
- PMID: 25328815
- PMCID: PMC4198944
- DOI: 10.4172/2155-6113.S6-002
The Use of Ultra-Sensitive Molecular Assays in HIV Cure-Related Research
Abstract
Ultra-sensitive laboratory assays based on the Polymerase Chain Reaction (PCR) are playing an increasingly important role in HIV cure-related research. This article reviews the different assays available and how they have evolved. There is a great need for their standardization and for the establishment of reference reagents and testing algorithms to evaluate potential HIV cure-related treatments.
Keywords: 2-LTR; Eradication; HIV; Nucleic acid tests; PCR; Proviral DNA; Standardization.
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References
-
- Rosenberg ES, Brennan CA, Claessens C, Constantine NT, Murphy G, et al. Criteria for Laboratory Testing and Diagnosis of Human Immunodeficiency Virus Infection; Approved Guideline. Vol. 31. Clinical and Laboratory Standards Institute; Wayne, Pennsylvania: 2011. pp. 1–89.
-
- Lewin SR, Rouzioux C. HIV cure and eradication: how will we get from the laboratory to effective clinical trials? AIDS. 2011;25:885–897. - PubMed
-
- Casabianca A, Gori C, Orlandi C, Forbici F, Federico Perno C, et al. Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay. Mol Cell Probes. 2007;21:368–378. - PubMed
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