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. 2014 Oct 17;9(10):e110747.
doi: 10.1371/journal.pone.0110747. eCollection 2014.

Lipidomic assessment of plasma and placenta of women with early-onset preeclampsia

Henri Augusto Korkes  1 , Nelson Sass  1 , Antonio F Moron  2 , Niels Olsen S Câmara  3 , Tatiana Bonetti  4 , Ana Sofia Cerdeira  5 , Ismael Dale Cotrim Guerreiro Da Silva  4 , Leandro De Oliveira  6
Affiliations

Lipidomic assessment of plasma and placenta of women with early-onset preeclampsia

Henri Augusto Korkes et al. PLoS One. .

Abstract

Introduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.

Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.

Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.

Results: Approximately 200 lipid signals were quantified. The most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p<0.0001) and PK04 (p<0.0001).

Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Representative lipid spectrum showing signals in plasma samples of control and preeclamptic patients.
The signals were identified between the lipid tracks acquisition from 600 to 1200 m/z.
Figure 2
Figure 2. Lipid composition detected in plasma from control and preeclamptic patients.
Comparison of relative distribution of main class of lipids in plasma of normal (A) and preeclamptic (B) patients, established by the area of the peaks obtained for the main lipids identified. N = 10 in each group. [GP03]-(PS): Glycerophosphoserines or Phosphatidylserines; [GP02]-(PEt): Glycerophosphoethanolamines or Phosphatidylethonolamines; [PK12]-(FLV): Flavonoids; [GP01]-(PC): Glycerophosphocholines or Phosphatidylcholines.
Figure 3
Figure 3. Lipid composition detected in placenta from control and preeclamptic women.
Comparison of relative distribution of main class of lipids in placenta of normal (A) and preeclamptic (B) patients, established by the area of the peaks obtained for the main lipids identified. N = 10 in each group [GP03]-(PS): Glycerophosphoserines or Phosphatidylserines; [PK04] =  Macrolide and lactone polyketides; [PK12]-(FLV): Flavonoids; [GP02]-(PEt): Glycerophosphoethanolamines or Phosphatidylethonolamines; [GP06]-(PI): Glycerophosphoinositols or Phosphatidylinositols; [GL02]-(DG): Diradylglycerols; [GP04]-(PG): Glycerophosphoglycerols or Phosphatidylglycerols.
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References

    1. Rana S, Cerdeira AS, Wenger J, Salahuddin S, Lim KH, et al. (2012) Plasma concentrations of soluble endoglin versus standard evaluation in patients with suspected preeclampsia. PLoS One 7: e48259. - PMC - PubMed
    1. Borzychowski AM, Sargent IL, Redman CW (2006) Inflammation and pre-eclampsia. Semin Fetal Neonatal Med 11: 309–316. - PubMed
    1. de Oliveira LG, Karumanchi A, Sass N (2010) [Preeclampsia: oxidative stress, inflammation and endothelial dysfunction]. Rev Bras Ginecol Obstet 32: 609–616. - PubMed
    1. Ghulmiyyah L, Sibai B (2012) Maternal mortality from preeclampsia/eclampsia. Semin Perinatol 36: 56–59. - PubMed
    1. Bellamy L, Casas JP, Hingorani AD, Williams DJ (2007) Pre-eclampsia and risk of cardiovascular disease and cancer in later life: systematic review and meta-analysis. BMJ 335: 974. - PMC - PubMed

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