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Review
. 2015 Jan;17(1):93-101.
doi: 10.1208/s12248-014-9682-8. Epub 2014 Oct 18.

Emerging technologies to increase ligand binding assay sensitivity

Saloumeh K Fischer  1 , Alison Joyce, Mark Spengler, Tong-Yuan Yang, Yao Zhuang, Marianne Scheel Fjording, Alvydas Mikulskis
Affiliations
Review

Emerging technologies to increase ligand binding assay sensitivity

Saloumeh K Fischer et al. AAPS J. 2015 Jan.

Abstract

Ligand binding assays (LBAs) have been the method of choice for protein analyte measurements for more than four decades. Over the years, LBA methods have improved in sensitivity and achieved larger dynamic ranges by using alternative detection systems and new technologies. As a consequence, the landscape and application of immunoassay platforms has changed dramatically. The introduction of bead-based methods, coupled with single molecule detection standardization and the ability to amplify assay signals, has improved the sensitivity of many immunoassays, in some cases by several logs of magnitude. Three promising immunoassay platforms are described in this article: Single Molecule Counting (SMC™) from Singulex Inc, Single Molecule Arrays (Simoa™) from Quanterix Corporation, and Immuno-PCR (Imperacer®) from Chimera Biotec GmbH. These platforms have the potential to significantly improve immunoassay sensitivity and thereby address the bioanalytical needs and challenges faced during biopharmaceutical drug development.

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Figures

Fig. 1
Fig. 1
Singulex: Single Molecule Counting (SMC™) technology
Fig. 2
Fig. 2
Quanterix: Single Molecule Arrays (Simoa™) technology
Fig. 3
Fig. 3
Chimera: Imperacer® ImmunoPCR (IPCR) technology
See this image and copyright information in PMC

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