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. 2014 Oct 21;19(10):16909-24.
doi: 10.3390/molecules191016909.

Effects of yerba maté, a plant extract formulation ("YGD") and resveratrol in 3T3-L1 adipogenesis

Juliana C Santos  1 , Erica M F Gotardo  2 , Mitsue T Brianti  3 , Mahmood Piraee  4 , Alessandra Gambero  5 , Marcelo L Ribeiro  6
Affiliations

Effects of yerba maté, a plant extract formulation ("YGD") and resveratrol in 3T3-L1 adipogenesis

Juliana C Santos et al. Molecules. .

Abstract

We aimed to evaluate the in vitro effects of yerba maté, YGD (a herbal preparation containing yerba maté, guarana and damiana), and resveratrol on adipogenesis. The anti-adipogenic effects of yerba mate, YGD, resveratrol and YGD + resveratrol and yerba mate + resveratrol combinations were evaluated in 3T3-L1 cells by Oil Red staining, cellular triglyceride content, and PCR quantitative array. The results demonstrated that all of the tested compounds inhibited adipogenesis. Yerba maté extract significantly down-regulated the expression of genes that play an important role in regulating adipogenesis, such as Adig, Axin, Cebpa, Fgf10, Lep, Lpl, and Pparγ2. In addition, these genes, YGD also repressed Bmp2, Ccnd1, Fasn, and Srebf1. Resveratrol also modulated the expression of Adig, Bmp2, Ccnd1, C/EBPα, Fasn, Fgf10, Lep, Lpl, and Pparγ2. Moreover, resveratrol repressed Cebpb, Cdk4, Fgf2, and Klf15. The yerba maté extract and YGD up-regulated the expression of genes involved in inhibiting adipogenesis, such as Dlk-1, Klf2, and Ucp1. Resveratrol also induced the expression of Klf2 and Ucp1. In addition resveratrol modulated the Ddit3, Foxo1, Sirt1, and Sirt2. The combined effects of these compounds on gene expression showed similar results observed from individual treatments. Our data indicates that the synergy between the compounds favors the inhibition of adipogenesis.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of (yerba maté, guarana and damiana) YGD (A); yerba maté (B); resveratrol (C) or mixed compounds (D) on adipogenesis in 3T3-L1 cells according to Oil Red O Assay. * p < 0.05 and ** p < 0.01 compared with control group.
Figure 2
Figure 2
Effect of YGD (A); yerba maté (B); resveratrol (C) or mixed compounds (D) on adipogenesis in 3T3-L1 cells according to evaluation of triglyceride content. * p < 0.05 and ** p < 0.01 compared with control group.
Figure 3
Figure 3
Effect of yerba maté, YGD and resveratrol on 3T3-L1 differentiation. (A) Control; (B) YGD (100 μg/mL); (C) yerba maté (150 μg/mL); (D) resveratrol (10 μg/mL); (E) YGD + resveratrol (100:10 μg/mL); and (F) yerba maté + resveratrol (150:10 μg/mL). Adipogenic differentiation was assessed using Oil Red O staining for lipid droplets 100 ×.
See this image and copyright information in PMC

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