New parvovirus in child with unexplained diarrhea, Tunisia

Abstract

A divergent parvovirus genome was the only eukaryotic viral sequence detected in feces of a Tunisian child with unexplained diarrhea. Tusavirus 1 shared 44% and 39% identity with the nonstructural protein 1 and viral protein 1, respectively, of the closest genome, Kilham rat parvovirus, indicating presence of a new human viral species in the Protoparvovirus genus.

Figure

New parvovirus genome and phylogeny. A) Organization of the Tusavirus genome. Theoretical splicing for expression of viral protein (VP) 1 is shown. The alignment of the PLA2 regions of representatives of 5 protoparvovirus species show the calcium-binding region and catalytic residues in Tusavirus. Pairwise sliding window of percentage nucleotide similarity of Tusavirus aligns with the genetically closest Kilham rat parvovirus. B) Phylogenetic trees generated with nonstructural protein (NS) 1 and VP1 of Tusavirus and of the 5 International Committee on Taxonomy of Viruses–designated species in the Protoparvovirus genus. Scale bar indicatesamino acid substitutions per site. FPV, feline parvovirus; MEV, mink enteritis virus; CPV, canine parvovirus; RaPV, raccoon parvovirus; RPV1, rat parvovirus 1; MVMp, minute virus of mice, prototype; HaPV, hamster parvovirus; PPV-Kr, porcine parvovirus Kresse; Simian BuPV, Simian bufavirus; BuPV1, bufavirus 1; BuPV2, bufavirus 2; AMDV, Aleutian mink disease virus; GFAV, gray fox amdovirus; B19V-Lali, human parvovirus B19-Lali. Bootstrap values (based on 100 replicates) for each node are given if >70%.