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. 2014 Oct 15:8:1923-8.
doi: 10.2147/DDDT.S69791. eCollection 2014.

Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts

Magdalena Donejko  1 , Andrzej Przylipiak  1 , Edyta Rysiak  2 , Katarzyna Głuszuk  2 , Arkadiusz Surażyński  2
Affiliations

Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts

Magdalena Donejko et al. Drug Des Devel Ther. .

Abstract

Aim: The aim of this study was to evaluate the effect of caffeine on collagen biosynthesis in human skin fibroblasts and the influence of hyaluronic acid (HA) on this process.

Materials and methods: Collagen, [(3)H]-thymidine incorporation, and prolidase activity were measured in confluent human skin fibroblast cultures that had been treated with 1, 2, and 5 mM caffeine and with caffeine and 500 μg/mL HA. Western immunoblot analysis was performed to evaluate expression of β1-integrin receptor, insulin-like growth factor receptor phospho-Akt protein and mitogen-activated protein kinase (phospho-extracellular signal-regulated kinase).

Results: Caffeine inhibited collagen biosynthesis in a dose-dependent manner. The mechanism of this process was found at the level of prolidase activity. Caffeine significantly inhibited the enzyme activity. The addition of HA had no effect on collagen biosynthesis or prolidase activity in fibroblasts incubated with caffeine. Caffeine also had an inhibitory effect on DNA biosynthesis. HA, however, did not have any significant effect on this process. The inhibition of the expression of β1-integrin and insulin-like growth factor receptor in fibroblasts incubated with the caffeine indicates a possible mechanism of inhibition of collagen biosynthesis.

Conclusion: Caffeine reduces collagen synthesis in human cultured skin fibroblasts. HA did not have any significant protective effect on this process. This is the first study to our knowledge that reports caffeine-induced inhibition of collagen synthesis in human skin fibroblasts.

Keywords: caffeine; collagen; fibroblast; hyaluronic acid.

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Figures

Figure 1
Figure 1
Collagen biosynthesis measured as 5[3H]-proline incorporation into proteins susceptible to the action of bacterial collagenase in confluent human skin fibroblasts incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA). Note: Error bars represent ± standard deviation; n=3.
Figure 2
Figure 2
Prolidase activity in confluent human skin fibroblasts incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA). Note: Error bars represent ± standard deviation; n=3.
Figure 3
Figure 3
DNA biosynthesis measured as [3H]-thymidine incorporation into DNA in human skin fibroblasts (control) incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA). Note: Error bars represent ± standard deviation; n=3.
Figure 4
Figure 4
Western immunoblot analysis for β1-integrin (A), insulin-like growth factor I receptor (B), extracellular signal-regulated kinase (ERK1/2) (C), Akt kinase (D), and β-actin (E) in subconfluent human skin fibroblasts (control), cells treated with or without hyaluronic acid for 24 hours. Notes: 1: control, 2: caffeine 1 mM, 3: caffeine 2 mM, 4: caffeine 1 mM, 5: hyaluronic acid, 6: caffeine 1 mM and hyaluronic acid, 7: caffeine 2 mM and hyaluronic acid; 8: caffeine 5 mM and hyaluronic acid. Samples used for electrophoresis consisted of 20 μg protein of pooled cell extracts (8). The intensity of the bands staining was quantified by densitometry analysis.
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References

    1. Azam S, Hadi N, Khan NU, Hadi SM. Antioxidant and prooxidant properties of caffeine, theobromine and xanthine. Med Sci Monit. 2003;9(9):BR325–BR330. - PubMed
    1. Merighi S, Benini A, Mirandola P, et al. Caffeine Inhibits Adenosine-Induced Accumulation of Hypoxia-Inducible Factor-1α, Vascular Endothelial Growth Factor, and Interleukin-8 Expression in Hypoxic Human Colon Cancer Cells. Mol Pharmacol. 2007;72:395–406. - PubMed
    1. Bode AM, Dong Z. The enigmatic effects of caffeine in cell cycle and cancer. Cancer Lett. 2007;247(1):26–39. - PMC - PubMed
    1. Jafari M, Rabbani A. Studies on the mechanism of caffeine action in alveolar macrophages: caffeine elevates cyclic adenosine monophosphate level and prostaglandin synthesis. Metabolism. 2004;53(6):687–692. - PubMed
    1. Doyle W, Shide E, Thapa S, Chandrasekaran V. The effects of energy beverages on cultured cells. Food Chem Toxicol. 2012;50(10):3759–3768. - PubMed

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