doi: 10.1515/znc-1989-11-1213.
3'-Arylazido-beta-alanyl-2-azido ATP, a cross-linking photoaffinity label for F1ATPases
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- PMID: 2534345
- DOI: 10.1515/znc-1989-11-1213
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3'-Arylazido-beta-alanyl-2-azido ATP, a cross-linking photoaffinity label for F1ATPases
Z Naturforsch C J Biosci.
1989 Nov-Dec.
Free article
Abstract
The synthesis of the 3'-arylazido-2-azido ATP derivative 3'-O-(3-[N-(4-azido-2-nitrophenyl)-amino]propionyl)2-azido-adenosine 5'-triphosphate (2,3'-DiN3ATP) is described. The bifunctional photoreactive ATP analog is characterized spectroscopically. Photoaffinity labeling of F1ATPase from Micrococcus luteus by this analog results in the inactivation of the enzyme and in the formation of higher molecular weight cross-links, composed of alpha- and beta-subunits.
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