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. 2014 Oct 28;19(11):17381-99.
doi: 10.3390/molecules191117381.

Tissue-specific distribution of ginsenosides in different aged ginseng and antioxidant activity of ginseng leaf

Ying-Chun Zhang  1 , Geng Li  2 , Chao Jiang  3 , Bin Yang  4 , Hong-Jun Yang  5 , Hai-Yu Xu  6 , Lu-Qi Huang  7
Affiliations

Tissue-specific distribution of ginsenosides in different aged ginseng and antioxidant activity of ginseng leaf

Ying-Chun Zhang et al. Molecules. .

Abstract

The aim of this study was to systematically evaluate the effect of the cultivation year on the quality of different ginseng tissues. Qualitative and quantitative analyses of ginsenosides were conducted using a UPLC-UV-MS method. Eight main ginsenosides in three tissues (leaf, rhizome and main root) and four parts (periderm, phloem, cambium and xylem) of ginseng aged from 1 to 13 years were determined using a UPLC-PDA method. Additionally, the antioxidant capacities of ginseng leaves were analyzed by the DPPH, ABTS and HRSA methods. It was found that the contents of ginsenosides increased with cultivation years, causing a sequential content change of ginsenosides in an organ-specific manner: leaf > rhizome > main root. The ratio between protopanaxatriol (PPT, Rg1, Re and RF) and protopanaxadiol (PPD, Rb1, Rb2, RC and Rd) in the main root remained stable (about 1.0), while it increased in leaf from 1.37 to 3.14 and decreased in the rhizome from 0.99 to 0.72. The amount of ginsenosides accumulated in the periderm was 45.48 mg/g, which was more than twice as high compared with the other three parts. Furthermore, the antioxidant activities of ginseng leaves were measured as Trolox equivalents, showing that antioxidant activity increased along with time of cultivation. The results show that the best harvest time for shizhu ginseng is the fifth year of cultivation, and the root and rhizome could be used together within seven planting years for their similar PPT/PPD level. Besides, the quality of the ginseng products would be enhanced with the periderm. The ginseng leaf is rich in ginsenosides and has potential application for its antioxidant capacity.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
UPLC ginsenoside profile of P. ginseng: (A) Leaf; (B) Rhizome; (C) Main root; (D) Standards. Detection at 203 nm wavelength. Peaks: (1) ginsenoside-Rg1; (2) ginsenoside-Re; (3) ginsenoside-Ro; (4) ginsenoside-Rf; (5) ginsenoside-Rb1; (6) ginsenoside-Rc; (7) ginsenoside-Rb2 and (8) ginsenoside-Rd; (a) ginsenoside Ma-ginsenoside Re; (b) ginsenoside Rg2; (c) ginsenoside F5; (d) ginsenoside F3; (e) ginsenoside Rb3.
Figure 2
Figure 2
Chemical structures of eight ginsenosides.
Figure 3
Figure 3
The variation ratios of eight individual ginsenoside in leaf (L), rhizome (R) and main root (MR) of 1 to 13 year-old ginseng. Values show mean ± SE of three experiments performed in triplicate. (The defect points mean the components are not detected).
Figure 4
Figure 4
The distribution of eight ginsenosides in the periderm, phloem, cambium, and xylem of 5 year aged ginseng.
See this image and copyright information in PMC

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