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. 2015 Jan;168B(1):36-44.
doi: 10.1002/ajmg.b.32278. Epub 2014 Oct 29.

DNA extracted from saliva for methylation studies of psychiatric traits: evidence tissue specificity and relatedness to brain

Alicia K Smith  1 , Varun Kilaru, Torsten Klengel, Kristina B Mercer, Bekh Bradley, Karen N Conneely, Kerry J Ressler, Elisabeth B Binder
Affiliations

DNA extracted from saliva for methylation studies of psychiatric traits: evidence tissue specificity and relatedness to brain

Alicia K Smith et al. Am J Med Genet B Neuropsychiatr Genet. 2015 Jan.

Abstract

DNA methylation has become increasingly recognized in the etiology of psychiatric disorders. Because brain tissue is not accessible in living humans, epigenetic studies are most often conducted in blood. Saliva is often collected for genotyping studies but is rarely used to examine DNA methylation because the proportion of epithelial cells and leukocytes varies extensively between individuals. The goal of this study was to evaluate whether saliva DNA is informative for studies of psychiatric disorders. DNA methylation (HumanMethylation450 BeadChip) was assessed in saliva and blood samples from 64 adult African Americans. Analyses were conducted using linear regression adjusted for appropriate covariates, including estimated cellular proportions. DNA methylation from brain tissues (cerebellum, frontal cortex, entorhinal cortex, and superior temporal gyrus) was obtained from a publically available dataset. Saliva and blood methylation was clearly distinguishable though there was positive correlation overall. There was little correlation in CpG sites within relevant candidate genes. Correlated CpG sites were more likely to occur in areas of low CpG density (i.e., CpG shores and open seas). There was more variability in CpG sites from saliva than blood, which may reflect its heterogeneity. Finally, DNA methylation in saliva appeared more similar to patterns from each of the brain regions examined overall than methylation in blood. Thus, this study provides a framework for using DNA methylation from saliva and suggests that DNA methylation of saliva may offer distinct opportunities for epidemiological and longitudinal studies of psychiatric traits.

Keywords: EWAS; HumanMethylation450; biomarker; epigenetic; oragene.

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Figures

FIG. 1
FIG. 1
Tissue-specific DNA methylatlon patterns. Hierarchical clustering of each subject (x-axis) for all CpG sites (y-axis) segregates samples by tissue, with all saliva samples clustering to the left of the graph and all blood samples clustering to the right.
FIG. 2
FIG. 2
Correlation between saliva and blood. This histogram depicts the correiation coefficient comparing methylatian levels of blood and saliva for each of the CpG sites examined. Those that are shaded are positively correlated.
FIG. 3
FIG. 3
Genome-wide relationship between DNA methyl at ion in saliva, blood, and four brain regions. Euclidean distance was calculated for each tissue using (A) all CpG sites and [B) CpG sites that vary in saliva. For each plot, blood, and saliva were compared to each of the four brain regions. Smaller differences indicate more similarity between the compared tissues. CRBLM, cerebellum; FCTX, frontal cortex; EntCTX, entorhinal cortex; Gyrus, superior temporal gyrus.
FIG. 4
FIG. 4
Relationship between DNA methylation in saliva, blood, and brain regions for BDNF. Average DNA methylation at each CpG site Is plotted by tissue.
See this image and copyright information in PMC

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