Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jan;86(2):496-9.
doi: 10.1073/pnas.86.2.496.

Dramatic thermostabilization of yeast iso-1-cytochrome c by an asparagine----isoleucine replacement at position 57

G Das  1 , D R Hickey, D McLendon, G McLendon, F Sherman
Affiliations

Dramatic thermostabilization of yeast iso-1-cytochrome c by an asparagine----isoleucine replacement at position 57

G Das et al. Proc Natl Acad Sci U S A. 1989 Jan.

Abstract

Two Saccharomyces cerevisiae yeast mutants, cyc1-73 and cyc1-190, contain nonfunctional and presumably unstable forms of iso-1-cytochrome c due to Gly-34----Ser and His-38----Pro replacements, respectively. Second-site reversions that produced Asn-57----Ile replacements at least partially restored function, presumably by alleviating the instability of these two altered iso-1-cytochromes c. Introduction of the Ile-57 replacement by site-directed mutagenesis in an otherwise normal protein resulted in a 17 degrees C increase in the transition temperature (Tm), corresponding to over a 2-fold increase in the free energy change (delta G degrees) for thermal unfolding.

PubMed Disclaimer

References

    1. Biochemistry. 1986 Nov 4;25(22):6952-8 - PubMed
    1. Gene. 1986;45(3):333-8 - PubMed
    1. J Biol Chem. 1987 May 25;262(15):7125-31 - PubMed
    1. Annu Rev Biophys Biophys Chem. 1987;16:115-37 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6663-7 - PubMed

Publication types

LinkOut - more resources