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. 2014 Sep;18(Suppl 1):S45-8.
doi: 10.4103/0973-029X.141345.

Analysis of silver stained nucleolar organizing regions in odontogenic cysts and tumors

Md Prasanna  1 , Cr Charan  1 , Kranti Kiran Reddy Ealla  1 , V Surekha  1 , Ganesh Kulkarni  2 , Sandhya Gokavarapu  3
Affiliations

Analysis of silver stained nucleolar organizing regions in odontogenic cysts and tumors

Md Prasanna et al. J Oral Maxillofac Pathol. 2014 Sep.

Abstract

Objective: The present study aimed to investigate the probable differences in cell proliferation index of odontogenic cysts and tumors by means of a comparative silver stained nucleolar organizing region (AgNOR) quantification.

Study design: This descriptive cross-sectional study was done on archival paraffin blocks (n = 62), consisting of 10 odontogenic keratocysts, 10 dentigerous cysts, 10 radicular cysts, 10 conventional ameloblastomas, 10 adenomatoid odontogenic tumors, 10 calcifying epithelial odontogenic tumors and 2 ameloblasic carcinomas.

Results: The mean AgNOR count of odontogenic cysts was 1.709 and the benign odontogenic tumors was 1.862. Highest AgNOR count was recorded in odontogenic keratocyst and lowest was seen in radicular cyst. Statistically significant difference in AgNOR counts of ameloblastoma and adenomatoid odontogenic tumor, amelobalastoma and calcifying epithelial odontogenic tumor, benign odontogenic tumors and ameloblastic carcinoma were seen. AgNORs in ameloblastic carcinoma were more in number and more widely spread.

Conclusion: AgNOR technique may be considered a good indicator of cell proliferation in odontogenic cysts and tumors.

Keywords: Odontogenic cysts; odontogenic tumors; silver stained nucleolar organizing region.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Photomicrograph showing odontogenic tumor cells arranged in follicles with peripheral tall columnar cells (H&E ×10)
Figure 2
Figure 2
Photomicrograph showing odontogenic cystic lining with a supporting connective tissue wall (Dentigerous cyst, H&E, ×10)
Figure 3
Figure 3
Photomicrograph showing AgNor positivity in the nucleus of Ameloblastoma (IHC ×200)
Figure 4
Figure 4
Photomicrograph showing AgNor positivity in the nucleus of Dentigerous cyst (IHC ×100)
See this image and copyright information in PMC

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