Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Apr;63(2):139-46.
doi: 10.1007/s00005-014-0315-9. Epub 2014 Nov 1.

Differential inflammatory microRNA and cytokine expression in pulmonary sarcoidosis

Agnieszka Jazwa  1 , Lukasz Kasper, Maciej Bak, Mateusz Sobczak, Krzysztof Szade, Alicja Jozkowicz, Krzysztof Sladek, Jozef Dulak
Affiliations

Differential inflammatory microRNA and cytokine expression in pulmonary sarcoidosis

Agnieszka Jazwa et al. Arch Immunol Ther Exp (Warsz). 2015 Apr.

Abstract

Sarcoidosis is a granulomatous disease of unknown etiology. The disease has an important inflammatory and immune component; however, its immunopathogenesis is not completely understood. Recently, the role of microRNAs (miRNAs), the small non-coding RNAs, has attracted attention as both being involved in pathogenesis and serving as disease markers. Accordingly, changes in the expression of some miRNAs have been also associated with different autoimmune pathologies. However, not much is known about the role of miRNAs in sarcoidosis. Therefore, the aim of this study was to compare the level of expression of selected miRNAs in healthy individuals and patients with sarcoidosis. We detected significantly increased level of miR-34a in peripheral blood mononuclear cells isolated from sarcoidosis patients. Moreover, significantly up-regulated levels of interferon (IFN)-γ, IFN-γ inducible protein (IP-10) and vascular endothelial growth factor were detected in sera of patients when compared to healthy subjects. Our results add to a known inflammatory component in sarcoidosis. Changes in the levels of miR-34a may suggest its involvement in the pathology of this disease.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Serum cytokine and chemokine levels in diseased and healthy individuals. Luminex assay. a IFN-γ, b IFN-γ inducible protein (IP-10); c monokine induced by IFN-γ (MIG); d IL-8; e IL-6; f VEGF. *Significant difference between indicated groups (median) at p < 0.05 (Mann–Whitney test)
Fig. 2
Fig. 2
Relative expression of selected miRNAs in PBMC. a miR-34a, b miR-378. The level of both miRNAs was normalized to small nuclear U6. *Significant difference between indicated groups (median) at p < 0.05 (Mann–Whitney test)
See this image and copyright information in PMC

References

    1. Asano M, Minagawa T, Ohmichi M, et al. Detection of endogenous cytokines in sera or in lymph nodes obtained from patients with sarcoidosis. Clin Exp Immunol. 1991;84:92–96. doi: 10.1111/j.1365-2249.1991.tb08129.x. - DOI - PMC - PubMed
    1. Baughman RP, Culver DA, Judson MA. A concise review of pulmonary sarcoidosis. Am J Respir Crit Care Med. 2011;183:573–581. doi: 10.1164/rccm.201006-0865CI. - DOI - PMC - PubMed
    1. Bazan-Socha S, Zuk J, Plutecka H, et al. Collagen receptors alpha(1)beta(1) and alpha(2)beta(1) integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer. J Physiol Pharmacol. 2012;63:373–379. - PubMed
    1. Beirne P, Pantelidis P, Charles P, et al. Multiplex immune serum biomarker profiling in sarcoidosis and systemic sclerosis. Eur Respir J. 2009;34:1376–1382. doi: 10.1183/09031936.00028209. - DOI - PubMed
    1. Boldin MP, Taganov KD, Rao DS, et al. miR-146a is a significant brake on autoimmunity, myeloproliferation, and cancer in mice. J Exp Med. 2011;208:1189–1201. doi: 10.1084/jem.20101823. - DOI - PMC - PubMed

Publication types

LinkOut - more resources