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Review
. 2014 Nov 3:31:4A.7.1-15.
doi: 10.1002/9780470151808.sc04a07s31.

Laser-assisted generation of human induced pluripotent stem cells

Yang Liu  1 , Dongmei Wu, Donglin Lao, Cory Peterson, Kristi A Hohenstein Elliott, Evan Y Snyder
Affiliations
Review

Laser-assisted generation of human induced pluripotent stem cells

Yang Liu et al. Curr Protoc Stem Cell Biol. .

Abstract

This unit describes a procedure for generating human induced pluripotent stem cells (hiPSCs) using the Laser-Enabled Analysis and Processing (LEAP®) system, which combines high-throughput cell imaging with laser-mediated cell manipulation. Use of this system should not only improve the quality and uniformity of hiPSCs produced, but ultimately enable a more rapid, efficient, high-throughput, and automated production process.

Keywords: automated; high-throughput; iPSC generation; laser-assisted.

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