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. 1989 Mar;63(3):1338-44.
doi: 10.1128/JVI.63.3.1338-1344.1989.

Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine

G D Westrop  1 , K A Wareham, D M Evans, G Dunn, P D Minor, D I Magrath, F Taffs, S Marsden, M A Skinner, G C Schild, et al.
Affiliations

Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine

G D Westrop et al. J Virol. 1989 Mar.

Abstract

The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.

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