Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Mar;63(3):1404-7.
doi: 10.1128/JVI.63.3.1404-1407.1989.

Papillomavirus polypeptides E6 and E7 are zinc-binding proteins

M S Barbosa  1 , D R Lowy, J T Schiller
Affiliations

Papillomavirus polypeptides E6 and E7 are zinc-binding proteins

M S Barbosa et al. J Virol. 1989 Mar.

Abstract

Papillomavirus proteins E6 and E7 have Cys-X-X-Cys repeats which have been suggested to mediate zinc binding. We have developed a modification of an assay that detects zinc binding to proteins immobilized on filters. Using well-characterized metalloproteins, we show that, under reducing conditions, this assay distinguishes proteins that coordinate zinc through cysteine residues from those that bind the metal through other amino acids. Under these conditions, E6 and E7 polypeptides of human papillomavirus type 18 and bovine papillomavirus type 1 exhibited high-affinity zinc binding. Our results suggest that E6 and E7 are metalloproteins and may coordinate the metal ions through cysteine residues.

PubMed Disclaimer

References

    1. Nature. 1985 Mar 7-13;314(6006):111-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7880-4 - PubMed
    1. Science. 1986 Apr 25;232(4749):485-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4680-4 - PubMed
    1. Nature. 1987 Jan 1-7;325(6099):70-3 - PubMed

Publication types

MeSH terms

LinkOut - more resources