Selenoprotein P receptor from rat

Abstract

Radioreceptor assay technology was used to show the presence in the rat of a receptor that binds selenoprotein P, a selenocysteine-containing rat plasma protein. 75Se-labeled selenoprotein P bound to testis, kidney, and liver membranes. The binding was specific in that increasing amounts of partially-fractionated rat plasma specifically displaced the binding of 75Se-labeled selenoprotein P to testis membrane in a competitive manner. 75Se-labeled selenoprotein P binding was saturable in the presence of increasing amounts of testis membranes. The binding of 75Se-labeled selenoprotein P was optimal at about pH 4.2. Several proteins and blood fractions had little or no significant effect on binding of 75Se-labeled selenoprotein P to testis membranes. All plasma sources tested specifically displaced 75Se-labeled selenoprotein P from testis membrane, indicating that selenoprotein P-related proteins may be widespread in nature. The study indicated that selenoprotein P has a receptor and is involved in selenium transport.