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. 2015 Jan;43(Database issue):D298-9.
doi: 10.1093/nar/gku1046. Epub 2014 Nov 5.

REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

Richard J Roberts  1 , Tamas Vincze  2 , Janos Posfai  2 , Dana Macelis  2
Affiliations

REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

Richard J Roberts et al. Nucleic Acids Res. 2015 Jan.

Abstract

REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.

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Figures

Figure 1.
Figure 1.
Dotted lines signify methylated motifs awaiting gene assignment. The sharp increase starting in 2012 is the result of the introduction of SMRT sequencing.
See this image and copyright information in PMC

References

    1. Roberts R.J., Vincze T., Posfai J., Macelis D. REBASE–a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Res. 2010;38:D234–D236. - PMC - PubMed
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    1. Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A., Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T.F., Dybvig K., et al. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003;31:1805–1812. - PMC - PubMed
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