Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Oct;4(4):285-8.
doi: 10.4103/2225-4110.130950.

Total Phenol Content and In Vitro Antioxidant Potential of Helicanthus elastica (Desr.) Danser-A Less-explored Indian Mango Mistletoe

Koppala Narayana Sunil Kumar  1 , Ariyamuthu Saraswathy  2 , Swaminathan Amerjothy  3 , Thomas Susan  4 , Basaviah Ravishankar  5
Affiliations

Total Phenol Content and In Vitro Antioxidant Potential of Helicanthus elastica (Desr.) Danser-A Less-explored Indian Mango Mistletoe

Koppala Narayana Sunil Kumar et al. J Tradit Complement Med. 2014 Oct.

Abstract

Natural products are an important source of antioxidant molecules like tannins, phenolic compounds, flavonoids, etc., Helicanthus elastica (Desr.) Danser (Loranthaceae) is one such plant belonging to the category of mistletoe, and grows commonly on the mango trees in India. In the present study, an attempt has been made to assess the antioxidant properties of the plant. Ethanol extract of H. elastica growing on mango tree was studied using different in vitro models. Shade-dried whole plant material was extracted with ethanol by cold percolation. Fifty milligrams of the alcohol extract of H. elastica was weighed and dissolved in 10 ml of methanol. The resultant 5 mg/ml solution was suitably diluted to obtain different concentrations. Total phenol content, reducing power assay, and scavenging of free radicals like nitric oxide, hydroxyl, hydrogen peroxide, and 1,1-diphenyl-2-picrylhydrazyl were studied by standardized in vitro chemical methods using ascorbic acid as the standard. The total phenol content of the plant was found to be 1.89% w/w. The extract showed good reducing power as well as scavenging of free radicals (nitric oxide, hydroxyl, superoxide anion, and hydrogen peroxide) at concentrations ranging from 5 to 100 μg/ml. The study revealed the antioxidant potential of H. elastica.

Keywords: Ascorbic acid; Free radicals; Helicanthus elastica; Loranthaceae; Mango mistletoe; Total phenol content.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Reducing power assay of alcoholic extract of Helicanthus elastica
Figure 2
Figure 2
Nitric oxide radical scavenging activity of alcoholic extract of Helicanthus elastica
Figure 3
Figure 3
Hydroxyl radical scavenging activity of alcoholic extract of Helicanthus elastica
Figure 4
Figure 4
Superoxide anion radical scavenging activity of alcoholic extract of Helicanthus elastica
Figure 5
Figure 5
Hydrogen peroxide scavenging assay of alcoholic extract of Helicanthus elastica
Figure 6
Figure 6
DPPH scavenging assay of alcoholic extract (1 mg/ml) of Helicanthus elastica (BHT, butylated hydroxytoluene)
See this image and copyright information in PMC

References

    1. Halliwell B. Food derived antioxidants-evaluating their importance in food and in vivo. Food Sci Agric Chem. 1999;1:67–109.
    1. Sunil Kumar KN. PhD Thesis. Chennai: The University of Madras; 2011. Pharmacognostical, Phytochemical and Medicinal Activity Profile of Helicanthus elastica (Desr.) Danser (Mango Mistletoe)-Loranthaceae; pp. 45–63.
    1. 1st ed. Vol. 2. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH); 2008. The Ayurvedic Pharmacopoeia of India. Part II (Formulations) p. 263. Appendices 1 to 5.
    1. Oyaizee M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr. 1986;44:307–15.
    1. Garret DC. Japan: Chapman and Hall Ltd; 1964. The Quantitative analysis of Drugs; pp. 456–8.