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. 1989 Apr;86(7):2219-23.
doi: 10.1073/pnas.86.7.2219.

Mobility of the maize suppressor-mutator element in transgenic tobacco cells

P Masson  1 , N V Fedoroff
Affiliations

Mobility of the maize suppressor-mutator element in transgenic tobacco cells

P Masson et al. Proc Natl Acad Sci U S A. 1989 Apr.

Abstract

Maize Suppressor-mutator (Spm) transposable elements have been introduced into tobacco cells and a visual assay for Spm activity has been developed using a bacterial beta-glucuronidase gene. The Spm element is mobile in tobacco and can trans-activate excision of a transposition-defective Spm (dSpm) element either from a different site on the same transforming Ti plasmid or from a second plasmid. An Spm element expressed from the stronger cauliflower mosaic virus 35S promoter trans-activates transposition of a dSpm element earlier after its introduction into tobacco cells than an element expressed from its own promoter.

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