Kocuria rhizophila misidentified as Corynebacterium jeikeium and other errors caused by the Vitek MS system call for maintained microbiological competence in the era of matrix-assisted laser desorption ionization-time of flight mass spectrometry

Affiliations

¹ Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA kevin.alby@uphs.upenn.edu.
² Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Case Reports

Kevin Alby et al. J Clin Microbiol. 2015 Jan.

. 2015 Jan;53(1):360-1.

doi: 10.1128/JCM.02616-14. Epub 2014 Nov 12.

¹ Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA kevin.alby@uphs.upenn.edu.
² Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

No abstract available

Comment in

The infallible microbial identification test: does it exist?
van Belkum A, Welker M, Dunne WM Jr, Girard V. van Belkum A, et al. J Clin Microbiol. 2015 May;53(5):1786. doi: 10.1128/JCM.00281-15. J Clin Microbiol. 2015. PMID: 25883325 Free PMC article. No abstract available.
Reply to "The infallible microbial identification test: does it exist?".
Alby K, Glaser LJ, Edelstein PH. Alby K, et al. J Clin Microbiol. 2015 May;53(5):1787. doi: 10.1128/JCM.00310-15. J Clin Microbiol. 2015. PMID: 25883326 Free PMC article. No abstract available.

1. Alby K, Gilligan PH, Miller MB. 2013. Comparison of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry platforms for the identification of Gram-negative rods from patients with cystic fibrosis. J Clin Microbiol 51:3852–3854. doi:10.1128/JCM.01618-13. - DOI - PMC - PubMed
1. Jamal WY, Shahin M, Rotimi VO. 2013. Comparison of two matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry methods and API20AN for identification of clinically relevant anaerobic bacteria. J Med Microbiol 62:540–544. doi:10.1099/jmm.0.053256-0. - DOI - PubMed
1. Martiny D, Busson L, Wybo I, El Haj RA, Dediste A, Vandenberg O. 2012. Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol 50:1313–1325. doi:10.1128/JCM.05971-11. - DOI - PMC - PubMed
1. McElvania TeKippe E, Burnham CA. 26 June 2014, posting date Evaluation of the Bruker Biotyper and VITEK MS MALDI-TOF MS systems for the identification of unusual and/or difficult-to-identify microorganisms isolated from clinical specimens. Eur J Clin Microbiol Infect Dis PMID 24962194. doi:10.1007/s10096-014-2183-y. - DOI - PubMed
1. Lane DJ. 1991. 16S/23S rRNA sequencing, p 115–148. In Stackebrandt E, Goodfellow M (ed), Nucleic acid techniques in bacterial systematics. John Wiley and Sons, Chichester, United Kingdom.