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. 2015 Apr;135(4):1175-1177.
doi: 10.1038/jid.2014.484. Epub 2014 Nov 14.

Characterization of quiescent epithelial cells in mouse meibomian glands and hair follicle/sebaceous glands by immunofluorescence tomography

Geraint J Parfitt  1 , Mikhail Geyfman  1 , Yilu Xie  1 , James V Jester  2
Affiliations

Characterization of quiescent epithelial cells in mouse meibomian glands and hair follicle/sebaceous glands by immunofluorescence tomography

Geraint J Parfitt et al. J Invest Dermatol. 2015 Apr.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST

The authors state no conflict of interest.

Figures

Figure 1
Figure 1. Immunofluorescence tomography of the H2B-GFP/K5tTA mouse eyelid after doxycycline chase
(a) Hematoxylin and eosin (H & E) staining of BMMA-embedded mouse eyelid showing the meibomian gland (encircled white) and hair follicle/sebaceous gland (encircled yellow). Both the meibomian gland (red arrowhead) and the hair follicle (white arrowhead) exhibit populations of slow-cycling epithelial cells after 28-day doxycycline chase. (c) In the meibomian gland, the label-retaining cells (LRCs) are located at the ductule or ductal epithelium terminus where the acinus forms. (d) BLIMP1 is expressed in differentiated hair follicle and sebaceous gland (SG) and is unlikely to be a marker of sebocyte progenitors. The hair follicle LRCs (green) are restricted to the bulge region (b) and are absent in the SG. Scale bar =50 μm.
Figure 2
Figure 2. Quantification of GFP+ label–retaining cells (LRCs) and the expression of BLIMP1, SOX9, and CK6 in the meibomian gland and hair follicle/sebaceous gland after 56-day doxycycline chase
(a) The average total cell and (b) LRCs that express either SOX9, BLIMP1, or CK6 in the meibomian gland (blue) or hair follicle (orange). (c–h) Immuno-staining of meibomian gland (c, e, and g—encircled white), hair follicle (d, f, and h—encircled yellow), and sebaceous gland (f*—encircled red) showing co-localization of quiescent epithelial LRCs with (c and d) SOX9, (e and f), BLIMP1, and (g and h) CK6 in the H2B-GFP/K5tTA mouse after 56-day doxycycline chase. Scale bar =50 μm.
See this image and copyright information in PMC

References

    1. Cotsarelis G, Cheng SZ, Dong G, et al. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell. 1989;57:201–9. - PubMed
    1. Formeister EJ, Sionas AL, Lorance DK, et al. Distinct SOX9 levels differentially mark stem/ progenitor populations and enteroendocrine cells of the small intestine epithelium. Am J Physiol Gastrointest Liver Physiol. 2009;296:G1108–18. - PMC - PubMed
    1. Horsley V, O’Carroll D, Tooze R, et al. Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland. Cell. 2006;126:597–609. - PMC - PubMed
    1. Kadaja M, Keyes BE, Lin M, et al. SOX9: a stem cell transcriptional regulator of secreted niche signaling factors. Genes Dev. 2014;28:328–41. - PMC - PubMed
    1. Knop E, Knop N, Millar T, et al. The international workshop on meibomian gland dysfunction: report of the subcommittee on anatomy, physiology, and pathophysiology of the meibomian gland. Invest Ophthalmol Vis Sci. 2011;52:1938–78. - PMC - PubMed

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