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. 2014 Sep 15;7(10):6734-42.
eCollection 2014.

Expression and prognostic value of MAGE-A9 in laryngeal squamous cell carcinoma

Liang Han  1 , Bin Jiang  2 , Hao Wu  3 , Shu Zhang  4 , Xueguan Lu  5
Affiliations

Expression and prognostic value of MAGE-A9 in laryngeal squamous cell carcinoma

Liang Han et al. Int J Clin Exp Pathol. .

Abstract

Background: Melanoma-associated antigen (MAGE) family genes are reported to play important roles in the development of human cancers. However, the relationship between the expression of MAGE-A9 and clinicopathological characteristics in human laryngeal carcinoma remains unclear. This study aimed to examine the expression of MAGE-A9, and to evaluate the clinical significance of its expression in human laryngeal squamous cell carcinoma (LSCC).

Methods: Quantitative real-time reverse transcription-PCR (qPCR) and immunohistochemistry (IHC) were performed to characterize the expression of MAGE-A9 in LSCC tissues and tumor-adjacent normal tissues. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of patients with LSCC.

Results: The expression of MAGE-A9 was significantly higher in LSCC than in tumor-adjacent normal tissues. Cytoplasmic expression of MAGE-A9 was detected in 70 of 123 (56.9%) LSCC specimens. Levels of MAGE-A9 in LSCC were related to histopathological grade (P = 0.024). Kaplan-Meier survival and Cox regression analysis revealed that MAGE-A9 expression level and lymph node metastasis were independent prognostic factors of LSCC (P = 0.005; P = 0.001, respectively).

Conclusions: Our study suggests that MAGE-A9 expression is a prognostic biomarker for LSCC patients. High expression of MAGE-A9 indicates unfavorable survival outcome in LSCC patients.

Keywords: Laryngeal squamous cell carcinoma; MAGE-A9; prognosis.

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Figures

Figure 1
Figure 1
One-step quantitative reverse transcription-polymerase chain reaction (qPCR) was employed to evaluate MAGE-A9 mRNA expression levels in LSCC (Ca) compared with tumor adjacent tissue (N). Levels of MAGE-A9 mRNA in LSCC and tumor-adjacent normal tissues were 0.064 ± 0.0086 and 0.0123 ± 0.0045, respectively (t = 2.032, P = 0.028) after normalizing to β-actin.
Figure 2
Figure 2
Representative images of MAGE-A9 expression in LSCC and tumor-adjacent normal tissues. A1 and A2. Immunohistochemical staining for MAGE-A9 showing positive cytoplasmic staining in LSCC. Red arrow indicates positive cytoplasmic staining of MAGE-A9 in LSCC cells. B1 and B2. Positive cytoplasmic staining of MAGE-A9 in cancer cells and stromal cells. Red arrow indicates positive cytoplasmic staining of MAGE-A9 in LSCC cells; blue arrow indicates positive MAGE-A9 staining in stromal cells. C1 and C2. Negative staining of MAGE-A9 in tumor-adjacent normal tissue. Green arrow indicates negative MAGE-A9 staining in epithelial cells. Original magnification ×40 in A1, B1 and C1; ×400 in A2, B2 and C2.
Figure 3
Figure 3
Survival analysis of LSCC patients (n = 123) by Kaplan-Meier method. A. The overall survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). B. The overall survival rate in patients with positive lymph node metastasis (green line) was significantly lower than that of patients with negative lymph node metastasis (blue line).
See this image and copyright information in PMC

References

    1. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics, 2009. CA Cancer J Clin. 2009;59:225–49. - PubMed
    1. Wang DS, Pan CC, Lai HC, Huang JM. Expression of HMGA1 and Ezrin in laryngeal squamous cell carcinoma. Acta Otolaryngol. 2013;133:626–32. - PubMed
    1. Liu XK, Li Q, Xu LH, Hu LJ, Liao WG, Zhang XR, Liu ZM, Wu D, Zeng MS. Expression and clinical significance of SIAH in laryngeal squamous cell carcinoma. Med Oncol. 2013;30:485. - PubMed
    1. Boyle P, Ferlay J. Cancer incidence and mortality in Europe, 2004. Ann Oncol. 2005;16:481–8. - PubMed
    1. Ferlay J, Parkin DM, Steliarova-Foucher E. Estimates of cancer incidence and mortality in Europe in 2008. Eur J Cancer. 2010;46:765–81. - PubMed

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