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. 2014 Sep 15;7(10):6950-5.
eCollection 2014.

Helicobacter pylori regulates TLR4 and TLR9 during gastric carcinogenesis

Tian Rong Wang  1 , Jiang Chen Peng  1 , Yu Qi Qiao  1 , Ming Ming Zhu  1 , Di Zhao  1 , Jun Shen  1 , Zhi Hua Ran  1
Affiliations

Helicobacter pylori regulates TLR4 and TLR9 during gastric carcinogenesis

Tian Rong Wang et al. Int J Clin Exp Pathol. .

Abstract

Objective: To investigated the influence of H. pylori on TLR4 and TLR9 in gastric mucosa during gastric carcinogenesis.

Methods: Gastric biopsy specimens were taken from 148 patients and divided into five groups, including normal group (n = 10), chronic superficial gastritis group (n = 35), atrophy/intestinal metaplasia group (n = 35), dysplasia group (n = 34) and gastric carcinoma group (n = 34). Immunohistochemistry was used to detect the expression of TLR4 and TLR9. Geimsa staining and rapid urea test were used for determine H. pylori infection.

Results: TLR4 was detected in gastric epithelium and monocytes/macrophages in superficial gastritis, atrophy/intestinal metaplasia, dysplasia or carcinoma. TLR9 was mainly accentuated in monocytes/macrophages. TLR4 positive cells in epithelium and in monocytes/macrophages with H. pylori infection were much more than those without H. pylori infection. Similar results were also found in TLR9. When gastric epithelium was accompanied with H. pylori infection, TLR4 was significant higher in superficial gastritis and atrophy/intestinal metaplasia groups compared with dysplasia and carcinoma groups. When gastric epithelium was infected by H. pylori, TLR9 was significant higher in carcinoma group compared with superficial gastritis, atrophy/intestinal metaplasia and dysplasia. TLR4 and TLR9 show significant correlation with the severity of inflammation.

Conclusions: H. pylori infection was associated with increased expression of TLR4 and TLR9 in gastric mucosa. In superficial gastritis and atrophy/intestinal metaplasia the inflammation was predominately mediated by TLR4, while in gastric cancer the inflammation was mainly mediated by TLR9.

Keywords: Expression of Toll-like receptor; H. pylori; gastric carcinogenesis.

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Figures

Figure 1
Figure 1
Expression of TLR4 in epithelium and monocytes/macrophages in different lesions.
Figure 2
Figure 2
Expression of TLR4 in different gastric lesions with HP infection.
See this image and copyright information in PMC

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