Comparative Study
doi: 10.1016/0014-5793(89)81335-3.
Hormone-sensitive lipase is responsible for the neutral cholesterol ester hydrolase activity in macrophages
Affiliations
- PMID: 2541013
- DOI: 10.1016/0014-5793(89)81335-3
Item in Clipboard
Comparative Study
Hormone-sensitive lipase is responsible for the neutral cholesterol ester hydrolase activity in macrophages
FEBS Lett.
.
Free article
Abstract
Anti-hormone-sensitive lipase (HSL) immunoglobulin selectively immunoprecipitates a single 84 kDa 32P-phosphoprotein from macrophage homogenates previously phosphorylated by cyclic AMP-dependent protein kinase in the presence of [gamma-32P]ATP-Mg. This immunoglobulin also completely removes the neutral cholesterol ester hydrolase activity from macrophage homogenates. These data demonstrate that HSL is responsible for the neutral cholesterol ester hydrolase activity in macrophages and hence plays a key role in cholesterol metabolism in these cells.
Similar articles
-
Purification and control of bovine adrenal cortical cholesterol ester hydrolase and evidence for the activation of the enzyme by a phosphorylation.Eur J Biochem. 1977 Jan;72(2):223-33. doi: 10.1111/j.1432-1033.1977.tb11243.x. Eur J Biochem. 1977. PMID: 189999
-
Regulation of cholesterol ester hydrolase by cyclic AMP-dependent protein kinase.FEBS Lett. 1986 Jun 9;201(2):257-61. doi: 10.1016/0014-5793(86)80619-6. FEBS Lett. 1986. PMID: 3011511
-
Phosphorylation and activation of hormone-sensitive lipase in isolated macrophages.FEBS Lett. 1991 Feb 25;279(2):323-6. doi: 10.1016/0014-5793(91)80179-7. FEBS Lett. 1991. PMID: 1705900
-
Hormone-sensitive lipase--a multipurpose enzyme in lipid metabolism.Biochim Biophys Acta. 1990 Apr 9;1052(1):128-32. doi: 10.1016/0167-4889(90)90067-n. Biochim Biophys Acta. 1990. PMID: 2182129 Review. No abstract available.
-
Cholesterol ester hydrolase and sterol carrier proteins.Endocr Res. 1984-1985;10(3-4):473-505. doi: 10.1080/07435808409036512. Endocr Res. 1984. PMID: 6100253 Review.
Cited by
-
Sterol-mediated regulation of hormone-sensitive lipase in 3T3-L1 adipocytes.Lipids. 2003 Jul;38(7):743-50. doi: 10.1007/s11745-003-1122-9. Lipids. 2003. PMID: 14506837
-
Chemical screen to reduce sterol accumulation in Niemann-Pick C disease cells identifies novel lysosomal acid lipase inhibitors.Biochim Biophys Acta. 2009 Dec;1791(12):1155-65. doi: 10.1016/j.bbalip.2009.08.005. Epub 2009 Aug 20. Biochim Biophys Acta. 2009. PMID: 19699313 Free PMC article.
-
Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis.FASEB J. 2020 Aug;34(8):10731-10750. doi: 10.1096/fj.202000671R. Epub 2020 Jul 2. FASEB J. 2020. PMID: 32614098 Free PMC article.
-
Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections.Parasitol Res. 2013 Feb;112(2):501-9. doi: 10.1007/s00436-012-3159-4. Epub 2012 Nov 9. Parasitol Res. 2013. PMID: 23138474
-
Macrophage cholesteryl ester hydrolases and hormone-sensitive lipase prefer specifically oxidized cholesteryl esters as substrates over their non-oxidized counterparts.Biochem J. 2000 Nov 15;352 Pt 1(Pt 1):125-33. Biochem J. 2000. PMID: 11062065 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
