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. 2014 Nov;109(7):964-6.
doi: 10.1590/0074-0276130413.

Evaluation of the role of ATP-binding cassette transporters as a defence mechanism against temephos in populations of Aedes aegypti

Estelita Pereira Lima  1 , Marília Oliveira Fonseca Goulart  2 , Modesto Leite Rolim Neto  1
Affiliations

Evaluation of the role of ATP-binding cassette transporters as a defence mechanism against temephos in populations of Aedes aegypti

Estelita Pereira Lima et al. Mem Inst Oswaldo Cruz. 2014 Nov.

Abstract

The role of ATP-binding cassette (ABC) transporters in the efflux of the insecticide, temephos, was assessed in the larvae of Aedes aegypti. Bioassays were conducted using mosquito populations that were either susceptible or resistant to temephos by exposure to insecticide alone or in combination with sublethal doses of the ABC transporter inhibitor, verapamil (30, 35 and 40 μM). The best result in the series was obtained with the addition of verapamil (40 μM), which led to a 2x increase in the toxicity of temephos, suggesting that ABC transporters may be partially involved in conferring resistance to the populations evaluated.

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Figures

Fig. 1
Fig. 1. : immunological tests applied to samples of the studied individuals in rural communities in the state of Querétaro, Mexico. FeSOD: iron superoxide dismutase; IFA: indirect immunofluorescence assay; IHA: indirect haemagglutination assay; rELISA: recombinant ELISA.
Fig. 2
Fig. 2. : prevalence of Trypanosoma cruzi seropositivity according to age group. FeSOD: iron superoxide dismutase; IFA: indirect immunofluorescence assay; IHA: indirect haemagglutination assay; rELISA: recombinant ELISA.
Fig. 3
Fig. 3. : percentages of age groups in the studied population.
Fig. 4
Fig. 4. : percentage of reactive cases in the relation to the different assays. FeSOD: iron superoxide dismutase; IFA: indirect immunofluorescence assay; IHA: indirect haemagglutination assay; rELISA: recombinant ELISA.
See this image and copyright information in PMC

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