Experience with an enzyme immunoassay for serotyping human group A rotaviruses
- PMID: 2541171
- PMCID: PMC267369
- DOI: 10.1128/jcm.27.3.586-588.1989
Experience with an enzyme immunoassay for serotyping human group A rotaviruses
Abstract
An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.
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