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Review
. 2015 Mar;42(3):349-60.
doi: 10.1007/s10295-014-1541-1. Epub 2014 Nov 22.

An integrated biotechnology platform for developing sustainable chemical processes

Nelson R Barton  1 , Anthony P Burgard, Mark J Burk, Jason S Crater, Robin E Osterhout, Priti Pharkya, Brian A Steer, Jun Sun, John D Trawick, Stephen J Van Dien, Tae Hoon Yang, Harry Yim
Affiliations
Review

An integrated biotechnology platform for developing sustainable chemical processes

Nelson R Barton et al. J Ind Microbiol Biotechnol. 2015 Mar.

Abstract

Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process.

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