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. 2015 Jan;85(1):20-8.
doi: 10.1111/tan.12477. Epub 2014 Nov 24.

Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry

M Zhou  1 , D Gao, X Chai, J Liu, Z Lan, Q Liu, F Yang, Y Guo, J Fang, L Yang, D Du, L Chen, X Yang, M Zhang, H Zeng, J Lu, H Chen, X Zhang, S Wu, Y Han, J Tan, Z Cheng, C Huang, W Wang
Affiliations

Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry

M Zhou et al. Tissue Antigens. 2015 Jan.

Abstract

Next generation sequencing (NGS)-based human leukocyte antigen (HLA) typing was used for ultra large-scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79,000 samples were subjected to HLA genotyping at 4-digit allelic level without ambiguities for HLA-A, -B, -C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes per run), and cost reduced by 95% compared with that of Sanger-based typing. Two percent of randomly selected samples were quality control (QC) tested at 4-digit allelic level by the CMDP QC laboratory, yielded a concordance of 99.72%. These results demonstrate that NGS is a cost effective and valuable tool for HLA typing of registry donors.

Keywords: allelic level; cost effective; genotyping; high throughput; human leukocyte antigen; next generation sequencing.

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