Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jun;171(6):3549-52.
doi: 10.1128/jb.171.6.3549-3552.1989.

Requirement for autoinducer in transcriptional negative autoregulation of the Vibrio fischeri luxR gene in Escherichia coli

P V Dunlap  1 , J M Ray
Affiliations

Requirement for autoinducer in transcriptional negative autoregulation of the Vibrio fischeri luxR gene in Escherichia coli

P V Dunlap et al. J Bacteriol. 1989 Jun.

Abstract

The effect of a mutation in luxI (autoinducer synthetase gene) on transcription of luxR in the cloned Vibrio fischeri lux system (luxR, luxICDABE) was examined in Escherichia coli. For the luxI mutant, transcription from the luxR promoter (monitored with beta-galactosidase levels from a luxR::lacZ fusion, with LuxR supplied in trans) decreased fivefold, to levels of the luxI+ strain, only in the presence of added autoinducer. The results demonstrate that, as has been shown at the translational level, autoinducer is required for negative autoregulation of luxR at the transcriptional level.

PubMed Disclaimer

References

    1. J Bacteriol. 1988 Sep;170(9):4040-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4154-8 - PubMed
    1. Arch Microbiol. 1986 Oct;146(1):35-40 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
    1. J Bacteriol. 1985 Oct;164(1):45-50 - PubMed

Publication types

LinkOut - more resources