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. 1989 Apr;27(4):770-2.
doi: 10.1128/jcm.27.4.770-772.1989.

Increased detection of herpes simplex virus in MRC-5 cells treated with dimethyl sulfoxide and dexamethasone

P G West  1 , B Aldrich, R Hartwig, G J Haller
Affiliations

Increased detection of herpes simplex virus in MRC-5 cells treated with dimethyl sulfoxide and dexamethasone

P G West et al. J Clin Microbiol. 1989 Apr.

Abstract

Treatment of MRC-5 cells with dexamethasone, dimethyl sulfoxide, or a combination of the two enhanced the detection of herpes simplex virus by three- to fourfold in these cells. Fluorescent plaques were noticeably larger on cover slips treated with the enhancing agents. Low-positive clinical specimens were stored and tested in parallel in treated and untreated shell vials, and 6 to 40% of these stained positive in the treated cultures but not in the untreated controls. In standard tube cultures, cytopathic effect began earlier and was more extensive in treated tubes.

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