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. 1989 May;86(10):3599-603.
doi: 10.1073/pnas.86.10.3599.

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase

J Colicelli  1 , C Birchmeier, T Michaeli, K O'Neill, M Riggs, M Wigler
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Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase

J Colicelli et al. Proc Natl Acad Sci U S A. 1989 May.

Abstract

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.

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References

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    1. Proc Natl Acad Sci U S A. 1989 May;86(10):3604-8 - PubMed
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