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Review
. 2015 Mar;15(5-6):1026-38.
doi: 10.1002/pmic.201400310. Epub 2015 Jan 21.

Current peptidomics: applications, purification, identification, quantification, and functional analysis

David C Dallas  1 , Andres Guerrero, Evan A Parker, Randall C Robinson, Junai Gan, J Bruce German, Daniela Barile, Carlito B Lebrilla
Affiliations
Review

Current peptidomics: applications, purification, identification, quantification, and functional analysis

David C Dallas et al. Proteomics. 2015 Mar.

Abstract

Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body-and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.

Keywords: Functional prediction; MS; Peptidomics; Quantitation; Structure prediction; Systems biology.

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Conflict of interest statement

The authors have declared no conflict of interest.

References

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