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. 2015:1254:329-39.
doi: 10.1007/978-1-4939-2152-2_23.

Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA)

Antón Barreiro-Iglesias  1 , Michael I Shifman
Affiliations

Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA)

Antón Barreiro-Iglesias et al. Methods Mol Biol. 2015.

Abstract

Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord. This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury.

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