Genomics-Based Exploration of Virulence Determinants and Host-Specific Adaptations of Pseudomonas syringae Strains Isolated from Grasses

Abstract

The Pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. The bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. It also serves as a model organism for research on the Type III secretion system (T3SS) and plant-pathogen interactions. While most of the current work on this pathogen is either carried out on one of three model strains found on dicot plants with completely sequenced genomes or on isolates obtained from recent outbreaks, not much is known about strains isolated from grasses (Poaceae). Here, we use comparative genomics in order to identify putative virulence-associated genes and other Poaceae-specific adaptations in several newly available genome sequences of strains isolated from grass species. All strains possess only a small number of known Type III effectors, therefore pointing to the importance of non-Type III secreted virulence factors. The implications of this finding are discussed.

Figure 1

Maximum likelihood phylogenetic tree of the analyzed P. syringae strains. Pgy, P. syringae pv. glycinea; Pph, P. syringae pv. phaseolicola; Psy, P. syringae pv. syringae; Paf, P. syringae pv. atrofaciens; Pja, P. syringae pv. japonica; Ppa, P. syringae pv. panici; Por, P. syringae pv. oryzae; Pto, P. syringae pv. tomato; P. cannabina pv. alisalensis (Pcal).

Figure 2

Shared genes in the genomes of the nine analyzed Poaceae isolates.

Figure 3

Type III effector repertoires.

Figure 4

Distribution of phytotoxins among the analyzed strains.