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. 2014 Nov;76(11):1443-50.
doi: 10.1292/jvms.14-0061. Epub 2014 Aug 22.

Low-level laser therapy attenuates LPS-induced rats mastitis by inhibiting polymorphonuclear neutrophil adhesion

Yueqiang Wang  1 , Xianjing He, Dandan Hao, Debin Yu, Jianbin Liang, Yanpeng Qu, Dongbo Sun, Bin Yang, Keli Yang, Rui Wu, Jianfa Wang
Affiliations

Low-level laser therapy attenuates LPS-induced rats mastitis by inhibiting polymorphonuclear neutrophil adhesion

Yueqiang Wang et al. J Vet Med Sci. 2014 Nov.

Abstract

The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on a rat model of lipopolysaccharide (LPS)-induced mastitis and its underlying molecular mechanisms. The rat model of mastitis was induced by inoculation of LPS through the canals of the mammary gland. The results showed that LPS-induced secretion of IL-1β and IL-8 significantly decreased after LLLT (650 nm, 2.5 mW, 30 mW/cm(2)). LLLT also inhibited intercellular adhesion molecule-1 (ICAM-1) expression and attenuated the LPS-induced decrease of the expression of CD62L and increase of the expression of CD11b. Moreover, LLLT also suppressed LPS-induced polymorphonuclear neutrophils (PMNs) entering the alveoli of the mammary gland. The number of PMNs in the mammary alveolus and the myeloperoxidase (MPO) activity were decreased after LLLT. These results suggested that LLLT therapy is beneficial in decreasing the somatic cell count and improving milk nutritional quality in cows with an intramammary infection.

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Figures

Fig. 1.
Fig. 1.
Effect of LLLT on cytokine concentration. The levels of IL-1β, IL-8 and TNF-α in serum (n=8 in each group) were detected by ELISA. A: effect of LLLT on the IL-1β concentration. B: effect of LLLT on the IL-8 concentration. C: effect of LLLT on the TNF-α concentration. The values are presented as means ± SEM (n=8 in each group). Values with different superscript letters at the same time point are significantly different from one another: a versus b=P<0.05; a versus c=P<0.05; b versus c=P<0.01.
Fig. 2.
Fig. 2.
Effect of LLLT on CD62L and CD11b positive cell percentages in blood PMNs. A: effect of LLLT on the CD62L positive cell percentage in blood PMNs; B: effect of LLLT on CD11b positive cell percentage in blood PMN. The values are presented as means ± SEM (n=8 in each group). Values with different superscript letters at the same time point are significantly different from one another: a versus b=P<0.05.
Fig. 3.
Fig. 3.
Effect of LLLT on ICAM-1 and PECAM-1 gene expressions. A: effect of LLLT on ICAM-1 gene expression; B: effect of LLLT on PECAM-1 gene expression. The values are presented as means ± SEM (n=8 in each group). Values with different superscript letters at the same time point are significantly different to one another: a versus b, and a versus c=P<0.05; b versus c=P<0.01.
Fig. 4.
Fig. 4.
Histological changes in the mammary tissues from each group (HE staining, × 400). Mammary tissues (n=8 in each group) from each group were processed for histological evaluation at 24 hr after LPS infusion. Representative histological changes of mammary tissues from each group: control group A, LPS+LLLT group; B, LPS group; C, LLLT group; D, control group. The black arrow indicates an representative PMN.
Fig. 5.
Fig. 5.
Effect of LLLT on the number of PMNs in the mammary gland. The values are presented as means ± SEM (n=8 in each group). Values with different superscript letters at the same time point are significantly different from one another: a versus b=P<0.05; a versus c=P<0.01.
Fig. 6.
Fig. 6.
Effect of LLLT on MPO activity.
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References

    1. Aimbire F., Ligeiro de Oliveira A. P., Albertini R., Corrêa J. C., Ladeira de Campos C. B., Lyon J. P., Silva J. A., Jr, Costa M. S.2008. Low level laser therapy (LLLT) decreases pulmonary microvascular leakage, neutrophil influx and IL-1beta levels in airway and lung from rat subjected to LPS-induced inflammation. Inflammation 31: 189–197. doi: 10.1007/s10753-008-9064-4 - DOI - PubMed
    1. Alves A. C., Vieira R. D., Leal-Junior E. C., Dos Santos S. A., Ligeiro A. P., Albertini R., Junior J. A., de Carvalho P. D.2013. Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation. Arthritis Res. Ther. 15: R116. doi: 10.1186/ar4296 - DOI - PMC - PubMed
    1. Barham W., Sherrill T., Connelly L., Blackwell T. S., Yull F. E.2012. Intraductal injection of LPS as a mouse model of mastitis: signaling visualized via an NF-κB reporter transgenic. J. Vis. Exp. 67: e4030. - PMC - PubMed
    1. Beneduci A., Chidichimo G., Nappi M. C., Rossi R., Turco R., Lucifora G., Garrafa F.2007. Evaluation of the effects induced by near infrared low intensity laser radiation on bovines affected by subacute mastitis: a single-quarter foremilk sampling study. J. Anim. Vet. Adv. 6: 761–767.
    1. Bjordal J. M., Lopes-Martins R. A. B., Joensen J., Iversen V. V.2010. The anti-inflammatory mechanism of low level laser therapy and its relevance for clinical use in physiotherapy. Phys. Ther. Rev. 15: 286–293. doi: 10.1179/1743288X10Y.0000000001 - DOI

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