Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jan;9(1):183-186.
doi: 10.3892/etm.2014.2047. Epub 2014 Nov 4.

Correlation between ApoE gene polymorphisms and the occurrence of urolithiasis

Biao Qian  1 , Liying Zheng  2 , Qinzhang Wang  1 , Guofu Ding  1
Affiliations

Correlation between ApoE gene polymorphisms and the occurrence of urolithiasis

Biao Qian et al. Exp Ther Med. 2015 Jan.

Abstract

The aim of the present study was to investigate the correlation between apolipoprotein E (ApoE) gene polymorphisms and the occurrence of urolithiasis and dyslipidemia. A total of 180 Uyghur individuals, including 90 urolithiasis patients and 90 healthy controls, were enrolled in this study. The blood lipid profiles of the patients and controls were investigated and compared, and the composition of the urinary calculi was determined. The polymorphisms of the ApoE alleles were analyzed using polymerase chain reaction-restriction fragment length polymorphism analysis. Three common genotypes of the ApoE gene, E3/3, E3/4 and E4/4, were detected in the urolithiasis patients and control group. In the patient group, 28 patients with the E3/3 genotype (30.1%), 58 patients with the E3/4 genotype (64.4%) and four patients with the E4/4 genotype (4.5%) were identified. By contrast, in the control group, 52 patients with the E3/3 genotype (57.8%), 35 patients with the E3/4 genotype (38.9%) and three patients with the E4/4 genotype (3.3%) were identified. The frequency of the E3/4 genotype was found to be significantly higher in the patient group when compared with the control group (χ2=12.96; P<0.001). In addition, the frequency of the E4 allele was significantly higher in the patient group when compared with the control group (χ2= 6.61; P=0.025). In conclusion, the occurrence of urolithiasis was found to be associated with ApoE gene polymorphisms, and the E4 allele may be a potential susceptibility factor for urolithiasis.

Keywords: Uyghur population; apolipoprotein E gene polymorphism; dyslipidemia; urolithiasis.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Apolipoprotein E gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism. Following digestion with the restriction endonuclease, Hha I, the DNA fragments were run on a polyacrylamide gel and the representative electrophoresis results of the E3/4 (91, 72 and 48 bp), E3/3 (91 and 48 bp) and E4/4 (72 and 48 bp) genotypes are shown.
See this image and copyright information in PMC

References

    1. Gu Y, Wang GZ, He W, Zhang J, Yang JW, Yang F, Ye M. Analysis of relative factors of urinary stones between patients of urinary stone and the health people in Shanghai Pudong area. Lin Chang Mi Niao Wai Ke Za Zhi. 2011;26:702–705. (In Chinese)
    1. Li LD. A primer on causes, prevention and treatment of urolithiasis. Hang Kong Hang Tian Yi Xue Za Zhi. 2011;22:688–689. (In Chinese)
    1. Ye RG. Internal Medicine. 5th Edition. People’s Medical Publishing House; Beijing: 2002. Dyslipidemia and Dyslipoproteinemia; pp. 834–837.
    1. Wilkinson IB, Prasad K, Hall IR, et al. Increased central pulse pressure and augmentation index in subjects with hypercholesterolemia. J Am Coll Cardiol. 2002;39:1005–1011. doi: 10.1016/S0735-1097(02)01723-0. - DOI - PubMed
    1. Evans RM, Hui S, Perkins A, Lahiri DK, Poirier J, Farlow MR. Cholesterol and APOE genotype interact to influence Alzheimer disease progression. Neurology. 2004;62:1869–1871. doi: 10.1212/01.WNL.0000125323.15458.3F. - DOI - PubMed

LinkOut - more resources